Blocking, blocking peptide, DNA, extract, Gene, Goat, Guinea, Hamster, Mink, Monkey, Mouse, Plant, protein-DNA, Proteins, Reagents, RNA, UPA
Expression and purification of functional recombinant CUL2•RBX1 from E. coli
Cullin-2 (CUL2) primarily based cullin-RING ligases (CRL2s) comprise a household of ubiquitin E3 ligases that exist solely in multi-cellular organisms and are essential for mobile processes equivalent to embryogenesis and viral pathogenesis. CUL2 is the scaffold protein that binds one of the interchangeable substrate receptor modules, which consists of adaptor proteins and the substrate receptor protein. The VHL protein is a substrate receptor identified to focus on hypoxia-inducible issue α (HIF1α) for ubiquitination and degradation.
Because of its essential function within the ubiquitination of necessary mobile components equivalent to HIF1α, CRL2s have been investigated for his or her organic features and the event of novel therapeutics towards illnesses. Given the significance of CRL2s in organic and biomedical analysis, strategies that effectively produce functional CUL2 proteins will significantly facilitate research on the mechanism and regulation of CRL2s.
Here, we report two cost-effective programs for the expression and purification of recombinant human CUL2 from E. coli cells. The purified CUL2 proteins have been ~ 95% pure, may bind their substrate receptor modules, and have been enzymatically lively in transferring ubiquitin or ubiquitin-like protein to the corresponding substrate in in vitro assays. The introduced methodological developments will assist advance analysis in CRL2 perform and regulation.
Milligram scale expression, refolding, and purification of Bombyx mori cocoonase utilizing a recombinant E. coli system
Silk is one of probably the most versatile biomaterials with signature properties of excellent mechanical energy and flexibility. A possible avenue for growing extra environmentally pleasant silk manufacturing is to make use of the silk moth (Bombyx mori) cocoonase, this may on the similar time improve the likelihood for utilizing the byproduct, sericin, as a uncooked materials for different functions.
Cocoonase is a serine protease utilized by the silk moth to melt the cocoon to allow its escape after accomplished metamorphosis. Cocoonase selectively degrades the glue protein of the cocoon, sericin, with out affecting the silk-fiber made of the protein fibroin. Cocoonase might be recombinantly produced in E. coli, nevertheless, it’s solely discovered as insoluble inclusion our bodies.
To clear up this drawback and to have the ability to make the most of the advantages related to an E. coli primarily based expression system, we’ve developed a protocol that allows the manufacturing of soluble and functional protease within the milligram/liter scale. The core of the protocol is refolding of the protein in a buffer with a redox potential that’s optimized for formation of native and intramolecular di-sulfide bridges.
The redox potential was balanced with outlined concentrations of decreased and oxidized glutathione. This E coli primarily based manufacturing protocol will, along with construction dedication, additionally allow modification of cocoonase each in phrases of catalytic perform and stability. These components might be helpful parts within the growth of alternate silk manufacturing methodology.
Expression of Bacillus amyloliquefaciens transglutaminase in recombinant E. coli below the management of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations
We studied the expression of Bacillus amyloliquefaciens transglutaminase cloned in Escherichia coli BL21(DE3)pLysS harboring the plasmid pBAD/3C/bTGase, a bicistronic expression system, in bioreactor cultivation. Batch and fed-batch managed as DO-stat methods have been employed for the manufacturing of the recombinant enzyme. In 30 h-batch cultivations utilizing Terrific broth (TB), 6 g/L of biomass and 3.12 U/mgprotein of transglutaminase exercise have been obtained.
DO-stat fed-batch cultivations below the management of oxygen focus (DO-stat) utilizing TB as medium however fed with glucose allowed the increment in biomass formation (17.5 g/L) and enzyme exercise (6.43 U/mgprotein). DO-stat fed-batch utilizing mineral medium (M9) and fed with glucose below the identical situations produced even greater enzymatic exercise (9.14 U/mgprotein).
The pH impact was investigated, and one of the best enzymatic exercise may very well be noticed at pH 8. In all cultivations, the bicistronic system remained secure, with 100% of plasmid-bearing cells. These outcomes present that E. coli bearing bicistronic plasmid constructs to specific recombinant TGase may very well be cultivated in bioreactors below DO-stat fed-batch utilizing mineral medium and it’s a promising technique in future optimizations to provide this necessary enzyme.
Characterization of Recombinant E. coli Expressing a Novel Fucosidase from Bacillus cereus 2-Eight belonging to GH95 Family
Fucoidan oligosaccharides possesses numerous physicochemical and organic actions. Specific glycoside hydrolases are helpful instruments for degrading polysaccharides to provide oligosaccharides. In this examine, BcFucA, a novel fucosidase belonging to GH95 household from Bacillus cereus 2-8, was cloned into pET21a vector, expressed in E. coli BL21 (DE3) and characterised.
Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306) |
|||
| VAng-Lsx1092-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein. |
|||
Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306) |
|||
| VAng-Lsx1092-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein. |
|||
Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306) |
|||
| VAng-Lsx1092-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein. |
|||
AIDA cloning plasmid |
|||
| CSB-CL839294HU-10ug | Cusabio | 10ug | EUR 279.6 |
|
Description: A cloning plasmid for the AIDA gene. |
|||
Anti-AIDA antibody |
|||
| PAab00234 | Lifescience Market | 100 ug | EUR 426 |
anti- AIDA antibody |
|||
| FNab00234 | FN Test | 100µg | EUR 606.3 |
|
Description: Antibody raised against AIDA |
|||
pCMV-SPORT6-AIDA |
|||
| PVT13939 | Lifescience Market | 2 ug | EUR 469.2 |
Human pre-microRNA Expression Construct Lenti-miR-30c-1, for distribution in Taiwan |
|||
| PMIRH30c-1-AA-1 | SBI | Miniprep DNA | EUR 820.8 |
Human pre-microRNA Expression Construct Lenti-miR-306 |
|||
| PMIRH306PA-1 | SBI | Bacterial Streak | EUR 820.8 |
6-dTMR [6-Carboxy-4,7-dichlorortetramethylrhodamine] |
|||
| 306 | AAT Bioquest | 25 mg | EUR 4183.2 |
Recombinant Clostridium Tetani glsA Protein (aa 1-306) |
|||
| VAng-Lsx01630-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4974 |
|
Description: Clostridium Tetani Glutaminase, recombinant protein. |
|||
Recombinant Clostridium Tetani glsA Protein (aa 1-306) |
|||
| VAng-Lsx01630-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Clostridium Tetani Glutaminase, recombinant protein. |
|||
Recombinant Clostridium Tetani glsA Protein (aa 1-306) |
|||
| VAng-Lsx01630-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Clostridium Tetani Glutaminase, recombinant protein. |
|||
Recombinant Clostridium Tetani metA Protein (aa 1-306) |
|||
| VAng-Lsx01673-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4974 |
|
Description: Clostridium Tetani Homoserine O-succinyltransferase, recombinant protein. |
|||
Recombinant Clostridium Tetani metA Protein (aa 1-306) |
|||
| VAng-Lsx01673-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Clostridium Tetani Homoserine O-succinyltransferase, recombinant protein. |
|||
Recombinant Clostridium Tetani metA Protein (aa 1-306) |
|||
| VAng-Lsx01673-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Clostridium Tetani Homoserine O-succinyltransferase, recombinant protein. |
|||
Recombinant Pseudomonas Aeruginosa rdgC Protein (aa 1-306) |
|||
| VAng-Cr0108-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Pseudomonas Aeruginosa recombination-associated protein rdgC (rdgC), recombinant protein. |
|||
Recombinant Pseudomonas Aeruginosa rdgC Protein (aa 1-306) |
|||
| VAng-Cr0108-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Pseudomonas Aeruginosa recombination-associated protein rdgC (rdgC), recombinant protein. |
|||
Recombinant Pseudomonas Aeruginosa rdgC Protein (aa 1-306) |
|||
| VAng-Cr0108-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Pseudomonas Aeruginosa recombination-associated protein rdgC (rdgC), recombinant protein. |
|||
Recombinant Klebsiella Pneumoniae speB Protein (aa 1-306) |
|||
| VAng-Cr5458-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Klebsiella Pneumoniae (strain 342) Agmatinase (speB), recombinant protein. |
|||
Recombinant Klebsiella Pneumoniae speB Protein (aa 1-306) |
|||
| VAng-Cr5458-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Klebsiella Pneumoniae (strain 342) Agmatinase (speB), recombinant protein. |
|||
Recombinant Klebsiella Pneumoniae speB Protein (aa 1-306) |
|||
| VAng-Cr5458-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Klebsiella Pneumoniae (strain 342) Agmatinase (speB), recombinant protein. |
|||
Recombinant Treponema Pallidum TP_0910 Protein (aa 1-306) |
|||
| VAng-Cr1430-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Treponema Pallidum (strain Nichols) uncharacterized protein TP_0910 (TP_0910), recombinant protein. |
|||
Recombinant Treponema Pallidum TP_0910 Protein (aa 1-306) |
|||
| VAng-Cr1430-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Treponema Pallidum (strain Nichols) uncharacterized protein TP_0910 (TP_0910), recombinant protein. |
|||
Recombinant Treponema Pallidum TP_0910 Protein (aa 1-306) |
|||
| VAng-Cr1430-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Treponema Pallidum (strain Nichols) uncharacterized protein TP_0910 (TP_0910), recombinant protein. |
|||
Recombinant Clostridium Novyi pyrB Protein (aa 1-306) |
|||
| VAng-Lsx00086-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Clostridium Novyi (strain NT) Aspartate carbamoyltransferase, recombinant protein. |
|||
Recombinant Clostridium Novyi pyrB Protein (aa 1-306) |
|||
| VAng-Lsx00086-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Clostridium Novyi (strain NT) Aspartate carbamoyltransferase, recombinant protein. |
|||
Recombinant Clostridium Novyi pyrB Protein (aa 1-306) |
|||
| VAng-Lsx00086-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Clostridium Novyi (strain NT) Aspartate carbamoyltransferase, recombinant protein. |
|||
Recombinant Clostridium Novyi tsf Protein (aa 1-306) |
|||
| VAng-Lsx00188-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Clostridium Novyi (strain NT) Elongation factor Ts, recombinant protein. |
|||
Recombinant Clostridium Novyi tsf Protein (aa 1-306) |
|||
| VAng-Lsx00188-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Clostridium Novyi (strain NT) Elongation factor Ts, recombinant protein. |
|||
Recombinant Clostridium Novyi tsf Protein (aa 1-306) |
|||
| VAng-Lsx00188-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Clostridium Novyi (strain NT) Elongation factor Ts, recombinant protein. |
|||
Recombinant Klebsiella Pneumoniae rbn Protein (aa 1-306) |
|||
| VAng-Cr5269-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Klebsiella Pneumoniae (strain 342) Ribonuclease BN (rbn), recombinant protein. |
|||
Recombinant Klebsiella Pneumoniae rbn Protein (aa 1-306) |
|||
| VAng-Cr5269-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Klebsiella Pneumoniae (strain 342) Ribonuclease BN (rbn), recombinant protein. |
|||
Recombinant Klebsiella Pneumoniae rbn Protein (aa 1-306) |
|||
| VAng-Cr5269-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Klebsiella Pneumoniae (strain 342) Ribonuclease BN (rbn), recombinant protein. |
|||
Recombinant ASFV Ken-108 Protein (aa 1-306) |
|||
| VAng-0866Lsx-1mg | Creative Biolabs | 1 mg | EUR 5289.6 |
|
Description: African swine fever virus (isolate Pig/Kenya/KEN-50/1950) Uncharacterized protein CP312R (Ken-108), recombinant protein from E. coli. (Uniprot ID: P0CAD1) |
|||
Recombinant ASFV Ken-108 Protein (aa 1-306) |
|||
| VAng-0866Lsx-500g | Creative Biolabs | 500 µg | EUR 3525.6 |
|
Description: African swine fever virus (isolate Pig/Kenya/KEN-50/1950) Uncharacterized protein CP312R (Ken-108), recombinant protein from E. coli. (Uniprot ID: P0CAD1) |
|||
Recombinant ASFV Ken-108 Protein (aa 1-306) |
|||
| VAng-0866Lsx-50g | Creative Biolabs | 50 µg | EUR 2403.6 |
|
Description: African swine fever virus (isolate Pig/Kenya/KEN-50/1950) Uncharacterized protein CP312R (Ken-108), recombinant protein from E. coli. (Uniprot ID: P0CAD1) |
|||
Recombinant Yersinia Enterocolitica yopD Protein (aa 1-306) |
|||
| VAng-Wyb2018-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Yersinia Enterocolitica Protein yopD, recombinant protein. |
|||
Recombinant Yersinia Enterocolitica yopD Protein (aa 1-306) |
|||
| VAng-Wyb2018-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Yersinia Enterocolitica Protein yopD, recombinant protein. |
|||
Recombinant Yersinia Enterocolitica yopD Protein (aa 1-306) |
|||
| VAng-Wyb2018-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Yersinia Enterocolitica Protein yopD, recombinant protein. |
|||
Recombinant Streptococcus Pneumoniae coaA Protein (aa 1-306) |
|||
| VAng-Ly1054-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Streptococcus Pneumoniae Pantothenate kinase, recombinant protein. |
|||
Recombinant Campylobacter Jejuni arcB Protein (aa 1-306) |
|||
| VAng-Ly2014-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Campylobacter Jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) ornithine carbamoyltransferase (arcB), recombination protein. |
|||
Recombinant Campylobacter Jejuni arcB Protein (aa 1-306) |
|||
| VAng-Ly2014-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Campylobacter Jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) ornithine carbamoyltransferase (arcB), recombination protein. |
|||
Recombinant Campylobacter Jejuni arcB Protein (aa 1-306) |
|||
| VAng-Ly2014-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Campylobacter Jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) ornithine carbamoyltransferase (arcB), recombination protein. |
|||
Recombinant Caenorhabditis Elegans ZC21.6 Protein (aa 1-306) |
|||
| VAng-Ly3933-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Caenorhabditis Elegans uncharacterized protein ZC21.6 (ZC21.6), recombination protein. |
|||
Recombinant Caenorhabditis Elegans ZC21.6 Protein (aa 1-306) |
|||
| VAng-Ly3933-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Caenorhabditis Elegans uncharacterized protein ZC21.6 (ZC21.6), recombination protein. |
|||
Recombinant Caenorhabditis Elegans ZC21.6 Protein (aa 1-306) |
|||
| VAng-Ly3933-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Caenorhabditis Elegans uncharacterized protein ZC21.6 (ZC21.6), recombination protein. |
|||
Recombinant Brucella Abortus arcB Protein (aa 1-306) |
|||
| VAng-Lsx5119-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Brucella Abortus biovar 1 (strain 9-941) Arginase, recombinant protein. |
|||
Recombinant Brucella Abortus arcB Protein (aa 1-306) |
|||
| VAng-Lsx5119-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Brucella Abortus biovar 1 (strain 9-941) Arginase, recombinant protein. |
|||
Recombinant Brucella Abortus arcB Protein (aa 1-306) |
|||
| VAng-Lsx5119-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Brucella Abortus biovar 1 (strain 9-941) Arginase, recombinant protein. |
|||
Recombinant Brucella Suis arcB Protein (aa 1-306) |
|||
| VAng-Lsx5597-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Brucella Suis biovar 1 (strain 1330) Arginase, recombinant protein. |
|||
Recombinant Brucella Suis arcB Protein (aa 1-306) |
|||
| VAng-Lsx5597-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Brucella Suis biovar 1 (strain 1330) Arginase, recombinant protein. |
|||
Recombinant Brucella Suis arcB Protein (aa 1-306) |
|||
| VAng-Lsx5597-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Brucella Suis biovar 1 (strain 1330) Arginase, recombinant protein. |
|||
Recombinant Brucella Suis fmt Protein (aa 1-306) |
|||
| VAng-Lsx5690-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Brucella Suis biovar 1 (strain 1330) Methionyl-tRNA formyltransferase, recombinant protein. |
|||
Recombinant Brucella Suis fmt Protein (aa 1-306) |
|||
| VAng-Lsx5690-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Brucella Suis biovar 1 (strain 1330) Methionyl-tRNA formyltransferase, recombinant protein. |
|||
Recombinant Brucella Suis fmt Protein (aa 1-306) |
|||
| VAng-Lsx5690-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Brucella Suis biovar 1 (strain 1330) Methionyl-tRNA formyltransferase, recombinant protein. |
|||
Recombinant Campylobacter Concisus lpxK Protein (aa 1-306) |
|||
| VAng-Lsx5831-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Campylobacter Concisus Tetraacyldisaccharide 4'-kinase, recombinant protein. |
|||
Recombinant Campylobacter Concisus lpxK Protein (aa 1-306) |
|||
| VAng-Lsx5831-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Campylobacter Concisus Tetraacyldisaccharide 4'-kinase, recombinant protein. |
|||
Recombinant Campylobacter Concisus lpxK Protein (aa 1-306) |
|||
| VAng-Lsx5831-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Campylobacter Concisus Tetraacyldisaccharide 4'-kinase, recombinant protein. |
|||
Recombinant Campylobacter Jejuni argF Protein (aa 1-306) |
|||
| VAng-Lsx6191-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Campylobacter Jejuni Ornithine carbamoyltransferase, recombinant protein. |
|||
Recombinant Campylobacter Jejuni argF Protein (aa 1-306) |
|||
| VAng-Lsx6191-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Campylobacter Jejuni Ornithine carbamoyltransferase, recombinant protein. |
|||
Recombinant Campylobacter Jejuni argF Protein (aa 1-306) |
|||
| VAng-Lsx6191-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Campylobacter Jejuni Ornithine carbamoyltransferase, recombinant protein. |
|||
Recombinant Clostridium Botulinum murB Protein (aa 1-306) |
|||
| VAng-Lsx8338-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Clostridium Botulinum (strain Okra / Type B1) UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein. |
|||
Recombinant Clostridium Botulinum murB Protein (aa 1-306) |
|||
| VAng-Lsx8338-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Clostridium Botulinum (strain Okra / Type B1) UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein. |
|||
Recombinant Clostridium Botulinum murB Protein (aa 1-306) |
|||
| VAng-Lsx8338-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Clostridium Botulinum (strain Okra / Type B1) UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein. |
|||
Recombinant Clostridium Cellulolyticum metA Protein (aa 1-306) |
|||
| VAng-Lsx8962-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Clostridium Cellulolyticum Homoserine O-succinyltransferase, recombinant protein. |
|||
Recombinant Clostridium Cellulolyticum metA Protein (aa 1-306) |
|||
| VAng-Lsx8962-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Clostridium Cellulolyticum Homoserine O-succinyltransferase, recombinant protein. |
|||
Recombinant Clostridium Cellulolyticum metA Protein (aa 1-306) |
|||
| VAng-Lsx8962-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Clostridium Cellulolyticum Homoserine O-succinyltransferase, recombinant protein. |
|||
Recombinant Clostridium Difficile pyrB Protein (aa 1-306) |
|||
| VAng-Lsx9272-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Clostridium Difficile (strain 630) Aspartate carbamoyltransferase, recombinant protein. |
|||
Recombinant Clostridium Difficile pyrB Protein (aa 1-306) |
|||
| VAng-Lsx9272-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Clostridium Difficile (strain 630) Aspartate carbamoyltransferase, recombinant protein. |
|||
Recombinant Clostridium Difficile pyrB Protein (aa 1-306) |
|||
| VAng-Lsx9272-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Clostridium Difficile (strain 630) Aspartate carbamoyltransferase, recombinant protein. |
|||
Recombinant Clostridium Acetobutylicum tsf Protein (aa 1-306) |
|||
| VAng-Lsx7894-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Clostridium Acetobutylicum Elongation factor Ts, recombinant protein. |
|||
Recombinant Clostridium Acetobutylicum tsf Protein (aa 1-306) |
|||
| VAng-Lsx7894-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Clostridium Acetobutylicum Elongation factor Ts, recombinant protein. |
|||
Recombinant Clostridium Acetobutylicum tsf Protein (aa 1-306) |
|||
| VAng-Lsx7894-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Clostridium Acetobutylicum Elongation factor Ts, recombinant protein. |
|||
Recombinant Shigella Boydii speB Protein (aa 1-306) |
|||
| VAng-Lsx08865-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5289.6 |
|
Description: Shigella Boydii serotype 4 (strain Sb227) Agmatinase, recombinant protein. |
|||
Recombinant Shigella Boydii speB Protein (aa 1-306) |
|||
| VAng-Lsx08865-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Shigella Boydii serotype 4 (strain Sb227) Agmatinase, recombinant protein. |
|||
Recombinant Shigella Boydii speB Protein (aa 1-306) |
|||
| VAng-Lsx08865-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Shigella Boydii serotype 4 (strain Sb227) Agmatinase, recombinant protein. |
|||
Recombinant Shigella Sonnei cbpA Protein (aa 1-306) |
|||
| VAng-Lsx09571-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5289.6 |
|
Description: Shigella Sonnei (strain Ss046) Curved DNA-binding protein, recombinant protein. |
|||
Recombinant Shigella Sonnei cbpA Protein (aa 1-306) |
|||
| VAng-Lsx09571-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Shigella Sonnei (strain Ss046) Curved DNA-binding protein, recombinant protein. |
|||
Recombinant Shigella Sonnei cbpA Protein (aa 1-306) |
|||
| VAng-Lsx09571-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Shigella Sonnei (strain Ss046) Curved DNA-binding protein, recombinant protein. |
|||
Recombinant Shigella Sonnei gsiC Protein (aa 1-306) |
|||
| VAng-Lsx09738-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Shigella Sonnei (strain Ss046) Glutathione transport system permease protein gsiC, recombinant protein. |
|||
Recombinant Borrelia Garinii murB Protein (aa 1-306) |
|||
| VAng-Lsx2135-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Borrelia Garinii UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein. |
|||
Recombinant Borrelia Garinii murB Protein (aa 1-306) |
|||
| VAng-Lsx2135-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Borrelia Garinii UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein. |
|||
Recombinant Borrelia Garinii murB Protein (aa 1-306) |
|||
| VAng-Lsx2135-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Borrelia Garinii UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein. |
|||
Recombinant Mycoplasma Pneumoniae MPN_285 Protein (aa 1-306) |
|||
| VAng-Lsx04890-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Mycoplasma Pneumoniae (strain ATCC 29342 / M129) Putative type-1 restriction enzyme specificity protein MPN_285, recombinant protein. |
|||
Recombinant Mycoplasma Pneumoniae MPN_285 Protein (aa 1-306) |
|||
| VAng-Lsx04890-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Mycoplasma Pneumoniae (strain ATCC 29342 / M129) Putative type-1 restriction enzyme specificity protein MPN_285, recombinant protein. |
|||
Recombinant Mycoplasma Pneumoniae MPN_285 Protein (aa 1-306) |
|||
| VAng-Lsx04890-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Mycoplasma Pneumoniae (strain ATCC 29342 / M129) Putative type-1 restriction enzyme specificity protein MPN_285, recombinant protein. |
|||
Recombinant Pasteurella Multocida era Protein (aa 1-306) |
|||
| VAng-Cr6799-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Pasteurella Multocida (strain Pm70) GTPase Era (era), recombinant protein. |
|||
Recombinant Pasteurella Multocida era Protein (aa 1-306) |
|||
| VAng-Cr6799-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Pasteurella Multocida (strain Pm70) GTPase Era (era), recombinant protein. |
|||
Recombinant Pasteurella Multocida era Protein (aa 1-306) |
|||
| VAng-Cr6799-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Pasteurella Multocida (strain Pm70) GTPase Era (era), recombinant protein. |
|||
Recombinant Streptococcus Mutans coaA Protein (aa 1-306) |
|||
| VAng-Cr7674-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Streptococcus Mutans Pantothenate kinase (coaA), recombinant protein. |
|||
Recombinant Streptococcus Mutans coaA Protein (aa 1-306) |
|||
| VAng-Cr7674-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Streptococcus Mutans Pantothenate kinase (coaA), recombinant protein. |
|||
Recombinant Streptococcus Mutans coaA Protein (aa 1-306) |
|||
| VAng-Cr7674-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Streptococcus Mutans Pantothenate kinase (coaA), recombinant protein. |
|||
Recombinant Helicobacter Pylori hemC Protein (aa 1-306) |
|||
| VAng-Lsx3515-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 5212.8 |
|
Description: Helicobacter Pylori Porphobilinogen deaminase, recombinant protein. |
|||
Recombinant Helicobacter Pylori hemC Protein (aa 1-306) |
|||
| VAng-Lsx3515-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3528 |
|
Description: Helicobacter Pylori Porphobilinogen deaminase, recombinant protein. |
|||
Recombinant Helicobacter Pylori hemC Protein (aa 1-306) |
|||
| VAng-Lsx3515-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2404.8 |
|
Description: Helicobacter Pylori Porphobilinogen deaminase, recombinant protein. |
|||
Recombinant Escherichia coli gsiC Protein (aa 1-306) |
|||
| VAng-Lsx02415-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Glutathione transport system permease protein gsiC, recombinant protein. |
|||
Recombinant Escherichia coli lpxL Protein (aa 1-306) |
|||
| VAng-Lsx02472-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Lipid A biosynthesis lauroyl acyltransferase, recombinant protein. |
|||
Recombinant Escherichia coli lpxP Protein (aa 1-306) |
|||
| VAng-Lsx02474-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Protein Ddg, recombinant protein. |
|||
Recombinant Escherichia coli oppB Protein (aa 1-306) |
|||
| VAng-Lsx02544-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Oligopeptide transport system permease protein oppB, recombinant protein. |
|||
Recombinant Escherichia coli yfdH Protein (aa 1-306) |
|||
| VAng-Lsx02836-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Escherichia coli (strain K12) Bactoprenol glucosyl transferase homolog from prophage CPS-53, recombinant protein. |
|||
Recombinant Haemophilus Influenzae ddl Protein (aa 1-306) |
|||
| VAng-Lsx03206-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Haemophilus Influenzae (strain PittGG) D-alanine--D-alanine ligase, recombinant protein. |
|||
Recombinant Haemophilus Influenzae ddl Protein (aa 1-306) |
|||
| VAng-Lsx03206-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Haemophilus Influenzae (strain PittGG) D-alanine--D-alanine ligase, recombinant protein. |
|||
Recombinant Haemophilus Influenzae ddl Protein (aa 1-306) |
|||
| VAng-Lsx03206-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Haemophilus Influenzae (strain PittGG) D-alanine--D-alanine ligase, recombinant protein. |
|||
Recombinant Haemophilus Influenzae oppB Protein (aa 1-306) |
|||
| VAng-Lsx03584-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Haemophilus Influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) Oligopeptide transport system permease protein oppB, recombinant protein. |
|||
Recombinant Haemophilus Influenzae truB Protein (aa 1-306) |
|||
| VAng-Lsx03923-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Haemophilus Influenzae (strain 86-028NP) tRNA pseudouridine synthase B, recombinant protein. |
|||
Recombinant Haemophilus Influenzae truB Protein (aa 1-306) |
|||
| VAng-Lsx03923-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Haemophilus Influenzae (strain 86-028NP) tRNA pseudouridine synthase B, recombinant protein. |
|||
Recombinant Haemophilus Influenzae truB Protein (aa 1-306) |
|||
| VAng-Lsx03923-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Haemophilus Influenzae (strain 86-028NP) tRNA pseudouridine synthase B, recombinant protein. |
|||
Recombinant Shigella Dysenteriae cbpA Protein (aa 1-306) |
|||
| VAng-Lsx06787-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Curved DNA-binding protein, recombinant protein. |
|||
Recombinant Shigella Dysenteriae cbpA Protein (aa 1-306) |
|||
| VAng-Lsx06787-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Curved DNA-binding protein, recombinant protein. |
|||
Recombinant Shigella Dysenteriae cbpA Protein (aa 1-306) |
|||
| VAng-Lsx06787-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Curved DNA-binding protein, recombinant protein. |
|||
Recombinant Shigella Dysenteriae speB Protein (aa 1-306) |
|||
| VAng-Lsx07206-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Agmatinase, recombinant protein. |
|||
Recombinant Shigella Dysenteriae speB Protein (aa 1-306) |
|||
| VAng-Lsx07206-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Agmatinase, recombinant protein. |
|||
Recombinant Shigella Dysenteriae speB Protein (aa 1-306) |
|||
| VAng-Lsx07206-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Agmatinase, recombinant protein. |
|||
Recombinant Shigella Dysenteriae gsiC Protein (aa 1-306) |
|||
| VAng-Lsx07424-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Glutathione transport system permease protein gsiC, recombinant protein. |
|||
Recombinant Shigella Flexneri cbpA Protein (aa 1-306) |
|||
| VAng-Lsx07644-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Shigella Flexneri Curved DNA-binding protein, recombinant protein. |
|||
Recombinant Shigella Flexneri cbpA Protein (aa 1-306) |
|||
| VAng-Lsx07644-500gEcoli | Creative Biolabs | 500 µg (E. coli) | EUR 3525.6 |
|
Description: Shigella Flexneri Curved DNA-binding protein, recombinant protein. |
|||
Recombinant Shigella Flexneri cbpA Protein (aa 1-306) |
|||
| VAng-Lsx07644-50gEcoli | Creative Biolabs | 50 µg (E. coli) | EUR 2403.6 |
|
Description: Shigella Flexneri Curved DNA-binding protein, recombinant protein. |
|||
Recombinant Shigella Flexneri lpxP Protein (aa 1-306) |
|||
| VAng-Lsx07901-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Shigella Flexneri Protein Ddg, recombinant protein. |
|||
Recombinant Shigella Flexneri oppB Protein (aa 1-306) |
|||
| VAng-Lsx08004-inquire | Creative Biolabs | inquire | Ask for price |
|
Description: Shigella Flexneri Oligopeptide transport system permease protein oppB, recombinant protein. |
|||
Recombinant Shigella Flexneri speB Protein (aa 1-306) |
|||
| VAng-Lsx08183-1mgEcoli | Creative Biolabs | 1 mg (E. coli) | EUR 4976.4 |
|
Description: Shigella Flexneri Agmatinase, recombinant protein. |
|||
The protein consists of 1136 amino acid residues encoded by 3411 bases and has a molecular weight of 125.35 kDa. The optimum temperature and pH of this enzyme are 50°C and pH 4.0. In addition, this examine confirmed that the unknown perform area (encoding Lys261-Thr681) outlined as a linker is sort of necessary for its exercise. The obtained novel enzyme BcFucA will contribute to the efficient degradation of fucoidan and future industrial functions.