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Expression and purification of functional recombinant CUL2•RBX1 from E. coli

by Jacob
Cullin-2 (CUL2) primarily based cullin-RING ligases (CRL2s) comprise a household of ubiquitin E3 ligases that exist solely in multi-cellular organisms and are essential for mobile processes equivalent to embryogenesis and viral pathogenesis. CUL2 is the scaffold protein that binds one of the interchangeable substrate receptor modules, which consists of adaptor proteins and the substrate receptor protein. The VHL protein is a substrate receptor identified to focus on hypoxia-inducible issue α (HIF1α) for ubiquitination and degradation.
Because of its essential function within the ubiquitination of necessary mobile components equivalent to HIF1α, CRL2s have been investigated for his or her organic features and the event of novel therapeutics towards illnesses. Given the significance of CRL2s in organic and biomedical analysis, strategies that effectively produce functional CUL2 proteins will significantly facilitate research on the mechanism and regulation of CRL2s.
Here, we report two cost-effective programs for the expression and purification of recombinant human CUL2 from E. coli cells. The purified CUL2 proteins have been ~ 95% pure, may bind their substrate receptor modules, and have been enzymatically lively in transferring ubiquitin or ubiquitin-like protein to the corresponding substrate in in vitro assays. The introduced methodological developments will assist advance analysis in CRL2 perform and regulation.

Milligram scale expression, refolding, and purification of Bombyx mori cocoonase utilizing a recombinant E. coli system

Silk is one of probably the most versatile biomaterials with signature properties of excellent mechanical energy and flexibility. A possible avenue for growing extra environmentally pleasant silk manufacturing is to make use of the silk moth (Bombyx mori) cocoonase, this may on the similar time improve the likelihood for utilizing the byproduct, sericin, as a uncooked materials for different functions.
Cocoonase is a serine protease utilized by the silk moth to melt the cocoon to allow its escape after accomplished metamorphosis. Cocoonase selectively degrades the glue protein of the cocoon, sericin, with out affecting the silk-fiber made of the protein fibroin. Cocoonase might be recombinantly produced in E. coli, nevertheless, it’s solely discovered as insoluble inclusion our bodies.
To clear up this drawback and to have the ability to make the most of the advantages related to an E. coli primarily based expression system, we’ve developed a protocol that allows the manufacturing of soluble and functional protease within the milligram/liter scale. The core of the protocol is refolding of the protein in a buffer with a redox potential that’s optimized for formation of native and intramolecular di-sulfide bridges.
The redox potential was balanced with outlined concentrations of decreased and oxidized glutathione. This E coli primarily based manufacturing protocol will, along with construction dedication, additionally allow modification of cocoonase each in phrases of catalytic perform and stability. These components might be helpful parts within the growth of alternate silk manufacturing methodology.

Expression of Bacillus amyloliquefaciens transglutaminase in recombinant E. coli below the management of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations

We studied the expression of Bacillus amyloliquefaciens transglutaminase cloned in Escherichia coli BL21(DE3)pLysS harboring the plasmid pBAD/3C/bTGase, a bicistronic expression system, in bioreactor cultivation. Batch and fed-batch managed as DO-stat methods have been employed for the manufacturing of the recombinant enzyme. In 30 h-batch cultivations utilizing Terrific broth (TB), 6 g/L of biomass and 3.12 U/mgprotein of transglutaminase exercise have been obtained.
Expression and purification of functional recombinant CUL2•RBX1 from E. coli
DO-stat fed-batch cultivations below the management of oxygen focus (DO-stat) utilizing TB as medium however fed with glucose allowed the increment in biomass formation (17.5 g/L) and enzyme exercise (6.43 U/mgprotein). DO-stat fed-batch utilizing mineral medium (M9) and fed with glucose below the identical situations produced even greater enzymatic exercise (9.14 U/mgprotein).
The pH impact was investigated, and one of the best enzymatic exercise may very well be noticed at pH 8. In all cultivations, the bicistronic system remained secure, with 100% of plasmid-bearing cells. These outcomes present that E. coli bearing bicistronic plasmid constructs to specific recombinant TGase may very well be cultivated in bioreactors below DO-stat fed-batch utilizing mineral medium and it’s a promising technique in future optimizations to provide this necessary enzyme.

Characterization of Recombinant E. coli Expressing a Novel Fucosidase from Bacillus cereus 2-Eight belonging to GH95 Family

Fucoidan oligosaccharides possesses numerous physicochemical and organic actions. Specific glycoside hydrolases are helpful instruments for degrading polysaccharides to provide oligosaccharides. In this examine, BcFucA, a novel fucosidase belonging to GH95 household from Bacillus cereus 2-8, was cloned into pET21a vector, expressed in E. coli BL21 (DE3) and characterised.

Mouse Aida ELISA KIT
ELI-24374m 96 Tests
EUR 1038

AIDA ELISA KIT|Human
EF007672 96 Tests
EUR 826.8

Human AIDA ELISA KIT
ELI-11961h 96 Tests
EUR 988.8

Human AIDA shRNA Plasmid
20-abx962119
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse AIDA shRNA Plasmid
20-abx979944
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

AIDA Recombinant Protein (Human)
RP000832 100 ug Ask for price

AIDA Recombinant Protein (Mouse)
RP115019 100 ug Ask for price

AIDA ORF Vector (Human) (pORF)
ORF000278 1.0 ug DNA
EUR 114

Aida ORF Vector (Mouse) (pORF)
ORF038341 1.0 ug DNA
EUR 607.2

Aida sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4069402 1.0 ug DNA
EUR 184.8

AIDA sgRNA CRISPR Lentivector (Human) (Target 1)
K0062102 1.0 ug DNA
EUR 184.8

Polyclonal AIDA Antibody (C-term)
APR04233G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AIDA (C-term). This antibody is tested and proven to work in the following applications:

Aida sgRNA CRISPR Lentivector set (Mouse)
K4069401 3 x 1.0 ug
EUR 406.8

AIDA sgRNA CRISPR Lentivector set (Human)
K0062101 3 x 1.0 ug
EUR 406.8

AIDA 3'UTR GFP Stable Cell Line
TU050511 1.0 ml
EUR 1825.2

Aida 3'UTR GFP Stable Cell Line
TU151582 1.0 ml Ask for price

AIDA Protein Vector (Human) (pPM-C-HA)
PV001111 500 ng
EUR 394.8

AIDA Protein Vector (Mouse) (pPM-C-HA)
PV153364 500 ng
EUR 723.6

AIDA Protein Vector (Human) (pPB-C-His)
PV001109 500 ng
EUR 394.8

AIDA Protein Vector (Human) (pPB-N-His)
PV001110 500 ng
EUR 394.8

AIDA Protein Vector (Human) (pPM-C-His)
PV001112 500 ng
EUR 394.8

AIDA Protein Vector (Mouse) (pPB-C-His)
PV153362 500 ng
EUR 723.6

AIDA Protein Vector (Mouse) (pPB-N-His)
PV153363 500 ng
EUR 723.6

AIDA Protein Vector (Mouse) (pPM-C-His)
PV153365 500 ng
EUR 723.6

AIDA 3'UTR Luciferase Stable Cell Line
TU000511 1.0 ml
EUR 1825.2

Aida 3'UTR Luciferase Stable Cell Line
TU101582 1.0 ml Ask for price

AIDA Protein Vector (Human) (pPB-His-MBP)
PV320710 500 ng
EUR 394.8

AIDA Protein Vector (Human) (pPB-His-GST)
PV320711 500 ng
EUR 394.8

Axin Interactor, Dorsalization Associated (AIDA) Antibody
abx230234-100ug 100 ug
EUR 577.2

Axin Interactor, Dorsalization Associated (AIDA) Antibody
abx029668-400ul 400 ul
EUR 627.6

Axin Interactor, Dorsalization Associated (AIDA) Antibody
abx029668-80l 80 µl
EUR 343.2

Aida sgRNA CRISPR Lentivector (Mouse) (Target 2)
K4069403 1.0 ug DNA
EUR 184.8

Aida sgRNA CRISPR Lentivector (Mouse) (Target 3)
K4069404 1.0 ug DNA
EUR 184.8

AIDA sgRNA CRISPR Lentivector (Human) (Target 2)
K0062103 1.0 ug DNA
EUR 184.8

AIDA sgRNA CRISPR Lentivector (Human) (Target 3)
K0062104 1.0 ug DNA
EUR 184.8

Recombinant Human AIDA Protein, His, E.coli-2ug
QP10986-2ug 2ug
EUR 186

Recombinant Human AIDA Protein, His, E.coli-10ug
QP10986-10ug 10ug
EUR 241.2

Recombinant Human AIDA Protein, His, E.coli-100ug
QP10986-100ug 100ug
EUR 1513.2

Aida sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K4069406 1.0 ug DNA
EUR 200.4

AIDA sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K0062106 1.0 ug DNA
EUR 200.4

AIDA Protein Vector (Human) (pPM-N-D-C-HA)
PV320712 500 ng
EUR 662.4

AIDA Protein Vector (Human) (pPM-N-D-C-His)
PV320713 500 ng
EUR 662.4

Human Axin Interactor, Dorsalization Associated (AIDA) ELISA Kit
20-abx385671
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Axin Interactor, Dorsalization Associated (AIDA) ELISA Kit
abx388645-96tests 96 tests
EUR 1093.2

Aida ELISA Kit| Mouse Axin interactor, dorsalization-associated
EF014270 96 Tests
EUR 826.8

AIDA Antibody / Axin interaction partner and dorsalization antagonist
RQ7361 100ug
EUR 419

AIDA Axin Interactor Dorsalization Associated Human Recombinant Protein
PROTQ96BJ3 Regular: 10ug
EUR 380.4
Description: AIDA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 329 amino acids (1-306 a.a) and having a molecular mass of 37.4kDa. AIDA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Aida sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K4069405 3 x 1.0 ug
EUR 451.2

AIDA sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K0062105 3 x 1.0 ug
EUR 451.2

Dog Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E08A1338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E08A1338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E07A1338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E07A1338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E07A1338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E02A1338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E02A1338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E02A1338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E08A1338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E01A1338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E01A1338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E01A1338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E03A1338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E03A1338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E03A1338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E06A1338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E06A1338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E06A1338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E04A1338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E04A1338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E04A1338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E09A1338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E09A1338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E09A1338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Aida sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K4069407 1.0 ug DNA
EUR 200.4

Aida sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K4069408 1.0 ug DNA
EUR 200.4

AIDA sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K0062107 1.0 ug DNA
EUR 200.4

AIDA sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K0062108 1.0 ug DNA
EUR 200.4

Guinea pig Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E05A1338-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E05A1338-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Axin interactor, dorsalization associated protein(AIDA) ELISA kit
E05A1338-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Axin interactor, dorsalization associated protein(AIDA) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

anti-AIDA
YF-PA27612 50 ul
EUR 435.6
Description: Mouse polyclonal to AIDA

Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306)
VAng-Lsx1092-1mgEcoli 1 mg (E. coli)
EUR 5212.8
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein.

Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306)
VAng-Lsx1092-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein.

Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306)
VAng-Lsx1092-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein.

Recombinant Salmonella oppB Protein (aa 1-306)
VAng-Wyb0568-inquire inquire Ask for price
Description: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Oligopeptide transport system permease protein oppB, recombinant protein.

Recombinant Clostridium Novyi tsf Protein (aa 1-306)
VAng-Lsx00188-1mgEcoli 1 mg (E. coli)
EUR 5212.8
Description: Clostridium Novyi (strain NT) Elongation factor Ts, recombinant protein.

Recombinant Clostridium Novyi tsf Protein (aa 1-306)
VAng-Lsx00188-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Clostridium Novyi (strain NT) Elongation factor Ts, recombinant protein.

Recombinant Clostridium Novyi tsf Protein (aa 1-306)
VAng-Lsx00188-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Clostridium Novyi (strain NT) Elongation factor Ts, recombinant protein.

Recombinant Haemophilus Influenzae ddl Protein (aa 1-306)
VAng-Lsx03206-1mgEcoli 1 mg (E. coli)
EUR 4976.4
Description: Haemophilus Influenzae (strain PittGG) D-alanine--D-alanine ligase, recombinant protein.

Recombinant Haemophilus Influenzae ddl Protein (aa 1-306)
VAng-Lsx03206-500gEcoli 500 µg (E. coli)
EUR 3525.6
Description: Haemophilus Influenzae (strain PittGG) D-alanine--D-alanine ligase, recombinant protein.

Recombinant Haemophilus Influenzae ddl Protein (aa 1-306)
VAng-Lsx03206-50gEcoli 50 µg (E. coli)
EUR 2403.6
Description: Haemophilus Influenzae (strain PittGG) D-alanine--D-alanine ligase, recombinant protein.

Recombinant Clostridium Tetani glsA Protein (aa 1-306)
VAng-Lsx01630-1mgEcoli 1 mg (E. coli)
EUR 4974
Description: Clostridium Tetani Glutaminase, recombinant protein.

Recombinant Clostridium Tetani glsA Protein (aa 1-306)
VAng-Lsx01630-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Clostridium Tetani Glutaminase, recombinant protein.

Recombinant Clostridium Tetani glsA Protein (aa 1-306)
VAng-Lsx01630-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Clostridium Tetani Glutaminase, recombinant protein.

Recombinant Clostridium Tetani metA Protein (aa 1-306)
VAng-Lsx01673-1mgEcoli 1 mg (E. coli)
EUR 4974
Description: Clostridium Tetani Homoserine O-succinyltransferase, recombinant protein.

Recombinant Clostridium Tetani metA Protein (aa 1-306)
VAng-Lsx01673-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Clostridium Tetani Homoserine O-succinyltransferase, recombinant protein.

Recombinant Clostridium Tetani metA Protein (aa 1-306)
VAng-Lsx01673-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Clostridium Tetani Homoserine O-succinyltransferase, recombinant protein.

Recombinant Clostridium Novyi pyrB Protein (aa 1-306)
VAng-Lsx00086-1mgEcoli 1 mg (E. coli)
EUR 5212.8
Description: Clostridium Novyi (strain NT) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Clostridium Novyi pyrB Protein (aa 1-306)
VAng-Lsx00086-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Clostridium Novyi (strain NT) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Clostridium Novyi pyrB Protein (aa 1-306)
VAng-Lsx00086-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Clostridium Novyi (strain NT) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Campylobacter Concisus lpxK Protein (aa 1-306)
VAng-Lsx5831-1mgEcoli 1 mg (E. coli)
EUR 5212.8
Description: Campylobacter Concisus Tetraacyldisaccharide 4'-kinase, recombinant protein.

Recombinant Campylobacter Concisus lpxK Protein (aa 1-306)
VAng-Lsx5831-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Campylobacter Concisus Tetraacyldisaccharide 4'-kinase, recombinant protein.

Recombinant Campylobacter Concisus lpxK Protein (aa 1-306)
VAng-Lsx5831-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Campylobacter Concisus Tetraacyldisaccharide 4'-kinase, recombinant protein.

Recombinant Campylobacter Jejuni argF Protein (aa 1-306)
VAng-Lsx6191-1mgEcoli 1 mg (E. coli)
EUR 5212.8
Description: Campylobacter Jejuni Ornithine carbamoyltransferase, recombinant protein.

Recombinant Campylobacter Jejuni argF Protein (aa 1-306)
VAng-Lsx6191-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Campylobacter Jejuni Ornithine carbamoyltransferase, recombinant protein.

Recombinant Campylobacter Jejuni argF Protein (aa 1-306)
VAng-Lsx6191-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Campylobacter Jejuni Ornithine carbamoyltransferase, recombinant protein.

Recombinant Clostridium Cellulolyticum metA Protein (aa 1-306)
VAng-Lsx8962-1mgEcoli 1 mg (E. coli)
EUR 5212.8
Description: Clostridium Cellulolyticum Homoserine O-succinyltransferase, recombinant protein.

Recombinant Clostridium Cellulolyticum metA Protein (aa 1-306)
VAng-Lsx8962-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Clostridium Cellulolyticum Homoserine O-succinyltransferase, recombinant protein.

Recombinant Clostridium Cellulolyticum metA Protein (aa 1-306)
VAng-Lsx8962-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Clostridium Cellulolyticum Homoserine O-succinyltransferase, recombinant protein.

Recombinant Brucella Suis arcB Protein (aa 1-306)
VAng-Lsx5597-1mgEcoli 1 mg (E. coli)
EUR 5212.8
Description: Brucella Suis biovar 1 (strain 1330) Arginase, recombinant protein.

Recombinant Brucella Suis arcB Protein (aa 1-306)
VAng-Lsx5597-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Brucella Suis biovar 1 (strain 1330) Arginase, recombinant protein.

Recombinant Brucella Suis arcB Protein (aa 1-306)
VAng-Lsx5597-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Brucella Suis biovar 1 (strain 1330) Arginase, recombinant protein.

Recombinant Brucella Suis fmt Protein (aa 1-306)
VAng-Lsx5690-1mgEcoli 1 mg (E. coli)
EUR 5212.8
Description: Brucella Suis biovar 1 (strain 1330) Methionyl-tRNA formyltransferase, recombinant protein.

Recombinant Brucella Suis fmt Protein (aa 1-306)
VAng-Lsx5690-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Brucella Suis biovar 1 (strain 1330) Methionyl-tRNA formyltransferase, recombinant protein.

Recombinant Brucella Suis fmt Protein (aa 1-306)
VAng-Lsx5690-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Brucella Suis biovar 1 (strain 1330) Methionyl-tRNA formyltransferase, recombinant protein.

Recombinant Clostridium Botulinum murB Protein (aa 1-306)
VAng-Lsx8338-1mgEcoli 1 mg (E. coli)
EUR 5212.8
Description: Clostridium Botulinum (strain Okra / Type B1) UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein.

Recombinant Clostridium Botulinum murB Protein (aa 1-306)
VAng-Lsx8338-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Clostridium Botulinum (strain Okra / Type B1) UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein.

Recombinant Clostridium Botulinum murB Protein (aa 1-306)
VAng-Lsx8338-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Clostridium Botulinum (strain Okra / Type B1) UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein.

Recombinant Clostridium Difficile pyrB Protein (aa 1-306)
VAng-Lsx9272-1mgEcoli 1 mg (E. coli)
EUR 5212.8
Description: Clostridium Difficile (strain 630) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Clostridium Difficile pyrB Protein (aa 1-306)
VAng-Lsx9272-500gEcoli 500 µg (E. coli)
EUR 3528
Description: Clostridium Difficile (strain 630) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Clostridium Difficile pyrB Protein (aa 1-306)
VAng-Lsx9272-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Clostridium Difficile (strain 630) Aspartate carbamoyltransferase, recombinant protein.
The protein consists of 1136 amino acid residues encoded by 3411 bases and has a molecular weight of 125.35 kDa. The optimum temperature and pH of this enzyme are 50°C and pH 4.0. In addition, this examine confirmed that the unknown perform area (encoding Lys261-Thr681) outlined as a linker is sort of necessary for its exercise. The obtained novel enzyme BcFucA will contribute to the efficient degradation of fucoidan and future industrial functions.