Expression and purification of functional recombinant CUL2•RBX1 from E. coli

Expression and purification of functional recombinant CUL2•RBX1 from E. coli
July 22, 2021 0 Comments

Cullin-2 (CUL2) primarily based cullin-RING ligases (CRL2s) comprise a household of ubiquitin E3 ligases that exist solely in multi-cellular organisms and are essential for mobile processes equivalent to embryogenesis and viral pathogenesis. CUL2 is the scaffold protein that binds one of the interchangeable substrate receptor modules, which consists of adaptor proteins and the substrate receptor protein. The VHL protein is a substrate receptor identified to focus on hypoxia-inducible issue α (HIF1α) for ubiquitination and degradation.
Because of its essential function within the ubiquitination of necessary mobile components equivalent to HIF1α, CRL2s have been investigated for his or her organic features and the event of novel therapeutics towards illnesses. Given the significance of CRL2s in organic and biomedical analysis, strategies that effectively produce functional CUL2 proteins will significantly facilitate research on the mechanism and regulation of CRL2s.
Here, we report two cost-effective programs for the expression and purification of recombinant human CUL2 from E. coli cells. The purified CUL2 proteins have been ~ 95% pure, may bind their substrate receptor modules, and have been enzymatically lively in transferring ubiquitin or ubiquitin-like protein to the corresponding substrate in in vitro assays. The introduced methodological developments will assist advance analysis in CRL2 perform and regulation.

Milligram scale expression, refolding, and purification of Bombyx mori cocoonase utilizing a recombinant Ecoli system

Silk is one of probably the most versatile biomaterials with signature properties of excellent mechanical energy and flexibility. A possible avenue for growing extra environmentally pleasant silk manufacturing is to make use of the silk moth (Bombyx mori) cocoonase, this may on the similar time improve the likelihood for utilizing the byproduct, sericin, as a uncooked materials for different functions.
Cocoonase is a serine protease utilized by the silk moth to melt the cocoon to allow its escape after accomplished metamorphosis. Cocoonase selectively degrades the glue protein of the cocoon, sericin, with out affecting the silk-fiber made of the protein fibroin. Cocoonase might be recombinantly produced in E. coli, nevertheless, it’s solely discovered as insoluble inclusion our bodies.
To clear up this drawback and to have the ability to make the most of the advantages related to an E. coli primarily based expression system, we’ve developed a protocol that allows the manufacturing of soluble and functional protease within the milligram/liter scale. The core of the protocol is refolding of the protein in a buffer with a redox potential that’s optimized for formation of native and intramolecular di-sulfide bridges.
The redox potential was balanced with outlined concentrations of decreased and oxidized glutathione. This E coli primarily based manufacturing protocol will, along with construction dedication, additionally allow modification of cocoonase each in phrases of catalytic perform and stability. These components might be helpful parts within the growth of alternate silk manufacturing methodology.

Expression of Bacillus amyloliquefaciens transglutaminase in recombinant Ecoli below the management of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations

We studied the expression of Bacillus amyloliquefaciens transglutaminase cloned in Escherichia coli BL21(DE3)pLysS harboring the plasmid pBAD/3C/bTGase, a bicistronic expression system, in bioreactor cultivation. Batch and fed-batch managed as DO-stat methods have been employed for the manufacturing of the recombinant enzyme. In 30 h-batch cultivations utilizing Terrific broth (TB), 6 g/L of biomass and 3.12 U/mgprotein of transglutaminase exercise have been obtained.
 Expression and purification of functional recombinant CUL2•RBX1 from E. coli
DO-stat fed-batch cultivations below the management of oxygen focus (DO-stat) utilizing TB as medium however fed with glucose allowed the increment in biomass formation (17.5 g/L) and enzyme exercise (6.43 U/mgprotein). DO-stat fed-batch utilizing mineral medium (M9) and fed with glucose below the identical situations produced even greater enzymatic exercise (9.14 U/mgprotein).
The pH impact was investigated, and one of the best enzymatic exercise may very well be noticed at pH 8. In all cultivations, the bicistronic system remained secure, with 100% of plasmid-bearing cells. These outcomes present that E. coli bearing bicistronic plasmid constructs to specific recombinant TGase may very well be cultivated in bioreactors below DO-stat fed-batch utilizing mineral medium and it’s a promising technique in future optimizations to provide this necessary enzyme.

Characterization of Recombinant Ecoli Expressing a Novel Fucosidase from Bacillus cereus 2-Eight belonging to GH95 Family

Fucoidan oligosaccharides possesses numerous physicochemical and organic actions. Specific glycoside hydrolases are helpful instruments for degrading polysaccharides to provide oligosaccharides. In this examine, BcFucA, a novel fucosidase belonging to GH95 household from Bacillus cereus 2-8, was cloned into pET21a vector, expressed in E. coli BL21 (DE3) and characterised.

anti-AIDA

YF-PA27612 50 ul
EUR 363
Description: Mouse polyclonal to AIDA

Recombinant Salmonella oppB Protein (aa 1-306)

VAng-Wyb0568-inquire inquire Ask for price
Description: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Oligopeptide transport system permease protein oppB, recombinant protein.

Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306)

VAng-Lsx1092-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein.

Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306)

VAng-Lsx1092-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein.

Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306)

VAng-Lsx1092-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein.

Platelet Membrane Glycoprotein IIB Peptide (296-306)

A1018-1 1 mg
EUR 87
Description: Human alloantisera specific for either the Bak' or Bakb allele have been shown to react exclusively with the heavy chain of membrane glycoprotein (GP) Ilb.GPllb is composed of a 125 Kd heavy chain that is disulfide-linked to a 23 Kd light chain.

Recombinant ASFV Ken-108 Protein (aa 1-306)

VAng-0866Lsx-1mg 1 mg
EUR 4408
Description: African swine fever virus (isolate Pig/Kenya/KEN-50/1950) Uncharacterized protein CP312R (Ken-108), recombinant protein from E. coli. (Uniprot ID: P0CAD1)

Recombinant ASFV Ken-108 Protein (aa 1-306)

VAng-0866Lsx-500g 500 µg
EUR 2938
Description: African swine fever virus (isolate Pig/Kenya/KEN-50/1950) Uncharacterized protein CP312R (Ken-108), recombinant protein from E. coli. (Uniprot ID: P0CAD1)

Recombinant ASFV Ken-108 Protein (aa 1-306)

VAng-0866Lsx-50g 50 µg
EUR 2003
Description: African swine fever virus (isolate Pig/Kenya/KEN-50/1950) Uncharacterized protein CP312R (Ken-108), recombinant protein from E. coli. (Uniprot ID: P0CAD1)

Recombinant Clostridium Novyi tsf Protein (aa 1-306)

VAng-Lsx00188-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Clostridium Novyi (strain NT) Elongation factor Ts, recombinant protein.

Recombinant Clostridium Novyi tsf Protein (aa 1-306)

VAng-Lsx00188-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Clostridium Novyi (strain NT) Elongation factor Ts, recombinant protein.

Recombinant Clostridium Novyi tsf Protein (aa 1-306)

VAng-Lsx00188-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Clostridium Novyi (strain NT) Elongation factor Ts, recombinant protein.

Recombinant Pasteurella Multocida era Protein (aa 1-306)

VAng-Cr6799-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Pasteurella Multocida (strain Pm70) GTPase Era (era), recombinant protein.

Recombinant Pasteurella Multocida era Protein (aa 1-306)

VAng-Cr6799-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Pasteurella Multocida (strain Pm70) GTPase Era (era), recombinant protein.

Recombinant Pasteurella Multocida era Protein (aa 1-306)

VAng-Cr6799-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Pasteurella Multocida (strain Pm70) GTPase Era (era), recombinant protein.

Recombinant Streptococcus Mutans coaA Protein (aa 1-306)

VAng-Cr7674-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Streptococcus Mutans Pantothenate kinase (coaA), recombinant protein.

Recombinant Streptococcus Mutans coaA Protein (aa 1-306)

VAng-Cr7674-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Streptococcus Mutans Pantothenate kinase (coaA), recombinant protein.

Recombinant Streptococcus Mutans coaA Protein (aa 1-306)

VAng-Cr7674-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Streptococcus Mutans Pantothenate kinase (coaA), recombinant protein.

Recombinant Clostridium Novyi pyrB Protein (aa 1-306)

VAng-Lsx00086-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Clostridium Novyi (strain NT) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Clostridium Novyi pyrB Protein (aa 1-306)

VAng-Lsx00086-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Clostridium Novyi (strain NT) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Clostridium Novyi pyrB Protein (aa 1-306)

VAng-Lsx00086-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Clostridium Novyi (strain NT) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Haemophilus Influenzae truB Protein (aa 1-306)

VAng-Lsx03923-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Haemophilus Influenzae (strain 86-028NP) tRNA pseudouridine synthase B, recombinant protein.

Recombinant Haemophilus Influenzae truB Protein (aa 1-306)

VAng-Lsx03923-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Haemophilus Influenzae (strain 86-028NP) tRNA pseudouridine synthase B, recombinant protein.

Recombinant Haemophilus Influenzae truB Protein (aa 1-306)

VAng-Lsx03923-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Haemophilus Influenzae (strain 86-028NP) tRNA pseudouridine synthase B, recombinant protein.

Recombinant Pseudomonas Aeruginosa rdgC Protein (aa 1-306)

VAng-Cr0108-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Pseudomonas Aeruginosa recombination-associated protein rdgC (rdgC), recombinant protein.

Recombinant Pseudomonas Aeruginosa rdgC Protein (aa 1-306)

VAng-Cr0108-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Pseudomonas Aeruginosa recombination-associated protein rdgC (rdgC), recombinant protein.

Recombinant Pseudomonas Aeruginosa rdgC Protein (aa 1-306)

VAng-Cr0108-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Pseudomonas Aeruginosa recombination-associated protein rdgC (rdgC), recombinant protein.

Recombinant Shigella Flexneri lpxP Protein (aa 1-306)

VAng-Lsx07901-inquire inquire Ask for price
Description: Shigella Flexneri Protein Ddg, recombinant protein.

Recombinant Shigella Flexneri oppB Protein (aa 1-306)

VAng-Lsx08004-inquire inquire Ask for price
Description: Shigella Flexneri Oligopeptide transport system permease protein oppB, recombinant protein.

Recombinant Shigella Flexneri speB Protein (aa 1-306)

VAng-Lsx08183-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Shigella Flexneri Agmatinase, recombinant protein.

Recombinant Shigella Flexneri speB Protein (aa 1-306)

VAng-Lsx08183-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Shigella Flexneri Agmatinase, recombinant protein.

Recombinant Shigella Flexneri speB Protein (aa 1-306)

VAng-Lsx08183-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Shigella Flexneri Agmatinase, recombinant protein.

Recombinant Clostridium Tetani glsA Protein (aa 1-306)

VAng-Lsx01630-1mgEcoli 1 mg (E. coli)
EUR 4145
Description: Clostridium Tetani Glutaminase, recombinant protein.

Recombinant Clostridium Tetani glsA Protein (aa 1-306)

VAng-Lsx01630-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Clostridium Tetani Glutaminase, recombinant protein.

Recombinant Clostridium Tetani glsA Protein (aa 1-306)

VAng-Lsx01630-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Clostridium Tetani Glutaminase, recombinant protein.

Recombinant Clostridium Tetani metA Protein (aa 1-306)

VAng-Lsx01673-1mgEcoli 1 mg (E. coli)
EUR 4145
Description: Clostridium Tetani Homoserine O-succinyltransferase, recombinant protein.

Recombinant Clostridium Tetani metA Protein (aa 1-306)

VAng-Lsx01673-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Clostridium Tetani Homoserine O-succinyltransferase, recombinant protein.

Recombinant Clostridium Tetani metA Protein (aa 1-306)

VAng-Lsx01673-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Clostridium Tetani Homoserine O-succinyltransferase, recombinant protein.

Recombinant Escherichia coli gsiC Protein (aa 1-306)

VAng-Lsx02415-inquire inquire Ask for price
Description: Escherichia coli (strain K12) Glutathione transport system permease protein gsiC, recombinant protein.

Recombinant Escherichia coli lpxL Protein (aa 1-306)

VAng-Lsx02472-inquire inquire Ask for price
Description: Escherichia coli (strain K12) Lipid A biosynthesis lauroyl acyltransferase, recombinant protein.

Recombinant Escherichia coli lpxP Protein (aa 1-306)

VAng-Lsx02474-inquire inquire Ask for price
Description: Escherichia coli (strain K12) Protein Ddg, recombinant protein.

Recombinant Escherichia coli oppB Protein (aa 1-306)

VAng-Lsx02544-inquire inquire Ask for price
Description: Escherichia coli (strain K12) Oligopeptide transport system permease protein oppB, recombinant protein.

Recombinant Escherichia coli yfdH Protein (aa 1-306)

VAng-Lsx02836-inquire inquire Ask for price
Description: Escherichia coli (strain K12) Bactoprenol glucosyl transferase homolog from prophage CPS-53, recombinant protein.

Recombinant Haemophilus Influenzae ddl Protein (aa 1-306)

VAng-Lsx03206-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Haemophilus Influenzae (strain PittGG) D-alanine--D-alanine ligase, recombinant protein.

Recombinant Haemophilus Influenzae ddl Protein (aa 1-306)

VAng-Lsx03206-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Haemophilus Influenzae (strain PittGG) D-alanine--D-alanine ligase, recombinant protein.

Recombinant Haemophilus Influenzae ddl Protein (aa 1-306)

VAng-Lsx03206-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Haemophilus Influenzae (strain PittGG) D-alanine--D-alanine ligase, recombinant protein.

Recombinant Haemophilus Influenzae oppB Protein (aa 1-306)

VAng-Lsx03584-inquire inquire Ask for price
Description: Haemophilus Influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) Oligopeptide transport system permease protein oppB, recombinant protein.

Recombinant Mycobacterium Tuberculosis cdsA Protein (aa 1-306)

VAng-Yyj0411-1mgEcoli 1 mg (E. coli)
EUR 4496
Description: Mycobacterium Tuberculosis Phosphatidate cytidylyltransferase (cdsA), recombinant protein.

Recombinant Treponema Pallidum TP_0910 Protein (aa 1-306)

VAng-Cr1430-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Treponema Pallidum (strain Nichols) uncharacterized protein TP_0910 (TP_0910), recombinant protein.

Recombinant Treponema Pallidum TP_0910 Protein (aa 1-306)

VAng-Cr1430-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Treponema Pallidum (strain Nichols) uncharacterized protein TP_0910 (TP_0910), recombinant protein.

Recombinant Treponema Pallidum TP_0910 Protein (aa 1-306)

VAng-Cr1430-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Treponema Pallidum (strain Nichols) uncharacterized protein TP_0910 (TP_0910), recombinant protein.

Recombinant Yersinia Enterocolitica yopD Protein (aa 1-306)

VAng-Wyb2018-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Yersinia Enterocolitica Protein yopD, recombinant protein.

Recombinant Yersinia Enterocolitica yopD Protein (aa 1-306)

VAng-Wyb2018-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Yersinia Enterocolitica Protein yopD, recombinant protein.

Recombinant Yersinia Enterocolitica yopD Protein (aa 1-306)

VAng-Wyb2018-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Yersinia Enterocolitica Protein yopD, recombinant protein.

Recombinant Caenorhabditis Elegans ZC21.6 Protein (aa 1-306)

VAng-Ly3933-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Caenorhabditis Elegans uncharacterized protein ZC21.6 (ZC21.6), recombination protein.

Recombinant Caenorhabditis Elegans ZC21.6 Protein (aa 1-306)

VAng-Ly3933-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Caenorhabditis Elegans uncharacterized protein ZC21.6 (ZC21.6), recombination protein.

Recombinant Caenorhabditis Elegans ZC21.6 Protein (aa 1-306)

VAng-Ly3933-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Caenorhabditis Elegans uncharacterized protein ZC21.6 (ZC21.6), recombination protein.

Recombinant Mycobacterium Avium ispE Protein(aa 1-306)

VAng-Yyj1246-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Mycobacterium Avium (strain 104) 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), recombinant protein.

Recombinant Mycobacterium Avium MAV_4442 Protein(aa 1-306)

VAng-Yyj1302-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Mycobacterium Avium (strain 104) Putative S-adenosyl-L-methionine-dependent methyltransferase MAV_4442 (MAV_4442), recombinant protein.

Recombinant Mycobacterium Leprae argF Protein(aa 1-306)

VAng-Yyj1504-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Mycobacterium Leprae Ornithine carbamoyltransferase (argF), recombinant protein.

Recombinant Mycobacterium Paratuberculosis MAP_4190c Protein(aa 1-306)

VAng-Yyj1650-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Mycobacterium Paratuberculosis Putative S-adenosyl-L-methionine-dependent methyltransferase MAP_4190c (MAP_4190c), recombinant protein.

Recombinant Mycobacterium Leprae rsmA Protein (aa 1-306)

VAng-Yyj2389-1mgEcoli 1 mg (E. coli)
EUR 4110
Description: Mycobacterium Leprae Ribosomal RNA small subunit methyltransferase A (rsmA), recombinant protein.

Recombinant Mycobacterium Leprae rsmA Protein (aa 1-306)

VAng-Yyj2389-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Mycobacterium Leprae Ribosomal RNA small subunit methyltransferase A (rsmA), recombinant protein.

Recombinant Mycobacterium Leprae rsmA Protein (aa 1-306)

VAng-Yyj2389-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Mycobacterium Leprae Ribosomal RNA small subunit methyltransferase A (rsmA), recombinant protein.

Recombinant Mycobacterium Avium ispE Protein (aa 1-306)

VAng-Yyj2581-1mgEcoli 1 mg (E. coli)
EUR 4145
Description: Mycobacterium Avium (strain 104) 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), recombinant protein.

Recombinant Mycobacterium Avium ispE Protein (aa 1-306)

VAng-Yyj2581-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Mycobacterium Avium (strain 104) 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), recombinant protein.

Recombinant Mycobacterium Avium ispE Protein (aa 1-306)

VAng-Yyj2581-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Mycobacterium Avium (strain 104) 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE), recombinant protein.

Recombinant Mycobacterium Avium MAV_4442 Protein (aa 1-306)

VAng-Yyj2631-1mgEcoli 1 mg (E. coli)
EUR 4145
Description: Mycobacterium Avium (strain 104) Putative S-adenosyl-L-methionine-dependent methyltransferase MAV_4442 (MAV_4442), recombinant protein.

Recombinant Mycobacterium Avium MAV_4442 Protein (aa 1-306)

VAng-Yyj2631-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Mycobacterium Avium (strain 104) Putative S-adenosyl-L-methionine-dependent methyltransferase MAV_4442 (MAV_4442), recombinant protein.

Recombinant Mycobacterium Avium MAV_4442 Protein (aa 1-306)

VAng-Yyj2631-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Mycobacterium Avium (strain 104) Putative S-adenosyl-L-methionine-dependent methyltransferase MAV_4442 (MAV_4442), recombinant protein.

Recombinant Mycoplasma Pneumoniae MPN_285 Protein (aa 1-306)

VAng-Lsx04890-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Mycoplasma Pneumoniae (strain ATCC 29342 / M129) Putative type-1 restriction enzyme specificity protein MPN_285, recombinant protein.

Recombinant Mycoplasma Pneumoniae MPN_285 Protein (aa 1-306)

VAng-Lsx04890-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Mycoplasma Pneumoniae (strain ATCC 29342 / M129) Putative type-1 restriction enzyme specificity protein MPN_285, recombinant protein.

Recombinant Mycoplasma Pneumoniae MPN_285 Protein (aa 1-306)

VAng-Lsx04890-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Mycoplasma Pneumoniae (strain ATCC 29342 / M129) Putative type-1 restriction enzyme specificity protein MPN_285, recombinant protein.

Recombinant Klebsiella Pneumoniae rbn Protein (aa 1-306)

VAng-Cr5269-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Klebsiella Pneumoniae (strain 342) Ribonuclease BN (rbn), recombinant protein.

Recombinant Klebsiella Pneumoniae rbn Protein (aa 1-306)

VAng-Cr5269-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Klebsiella Pneumoniae (strain 342) Ribonuclease BN (rbn), recombinant protein.

Recombinant Klebsiella Pneumoniae rbn Protein (aa 1-306)

VAng-Cr5269-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Klebsiella Pneumoniae (strain 342) Ribonuclease BN (rbn), recombinant protein.

Recombinant Klebsiella Pneumoniae speB Protein (aa 1-306)

VAng-Cr5458-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Klebsiella Pneumoniae (strain 342) Agmatinase (speB), recombinant protein.

Recombinant Klebsiella Pneumoniae speB Protein (aa 1-306)

VAng-Cr5458-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Klebsiella Pneumoniae (strain 342) Agmatinase (speB), recombinant protein.

Recombinant Klebsiella Pneumoniae speB Protein (aa 1-306)

VAng-Cr5458-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Klebsiella Pneumoniae (strain 342) Agmatinase (speB), recombinant protein.

Recombinant Shigella Boydii speB Protein (aa 1-306)

VAng-Lsx08865-1mgEcoli 1 mg (E. coli)
EUR 4408
Description: Shigella Boydii serotype 4 (strain Sb227) Agmatinase, recombinant protein.

Recombinant Shigella Boydii speB Protein (aa 1-306)

VAng-Lsx08865-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Shigella Boydii serotype 4 (strain Sb227) Agmatinase, recombinant protein.

Recombinant Shigella Boydii speB Protein (aa 1-306)

VAng-Lsx08865-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Shigella Boydii serotype 4 (strain Sb227) Agmatinase, recombinant protein.

Recombinant Shigella Sonnei cbpA Protein (aa 1-306)

VAng-Lsx09571-1mgEcoli 1 mg (E. coli)
EUR 4408
Description: Shigella Sonnei (strain Ss046) Curved DNA-binding protein, recombinant protein.

Recombinant Shigella Sonnei cbpA Protein (aa 1-306)

VAng-Lsx09571-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Shigella Sonnei (strain Ss046) Curved DNA-binding protein, recombinant protein.

Recombinant Shigella Sonnei cbpA Protein (aa 1-306)

VAng-Lsx09571-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Shigella Sonnei (strain Ss046) Curved DNA-binding protein, recombinant protein.

Recombinant Shigella Sonnei gsiC Protein (aa 1-306)

VAng-Lsx09738-inquire inquire Ask for price
Description: Shigella Sonnei (strain Ss046) Glutathione transport system permease protein gsiC, recombinant protein.

Recombinant Brucella Abortus arcB Protein (aa 1-306)

VAng-Lsx5119-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Brucella Abortus biovar 1 (strain 9-941) Arginase, recombinant protein.

Recombinant Brucella Abortus arcB Protein (aa 1-306)

VAng-Lsx5119-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Brucella Abortus biovar 1 (strain 9-941) Arginase, recombinant protein.

Recombinant Brucella Abortus arcB Protein (aa 1-306)

VAng-Lsx5119-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Brucella Abortus biovar 1 (strain 9-941) Arginase, recombinant protein.

Recombinant Brucella Suis arcB Protein (aa 1-306)

VAng-Lsx5597-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Brucella Suis biovar 1 (strain 1330) Arginase, recombinant protein.

Recombinant Brucella Suis arcB Protein (aa 1-306)

VAng-Lsx5597-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Brucella Suis biovar 1 (strain 1330) Arginase, recombinant protein.

Recombinant Brucella Suis arcB Protein (aa 1-306)

VAng-Lsx5597-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Brucella Suis biovar 1 (strain 1330) Arginase, recombinant protein.

Recombinant Brucella Suis fmt Protein (aa 1-306)

VAng-Lsx5690-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Brucella Suis biovar 1 (strain 1330) Methionyl-tRNA formyltransferase, recombinant protein.

Recombinant Brucella Suis fmt Protein (aa 1-306)

VAng-Lsx5690-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Brucella Suis biovar 1 (strain 1330) Methionyl-tRNA formyltransferase, recombinant protein.

Recombinant Brucella Suis fmt Protein (aa 1-306)

VAng-Lsx5690-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Brucella Suis biovar 1 (strain 1330) Methionyl-tRNA formyltransferase, recombinant protein.

Recombinant Campylobacter Concisus lpxK Protein (aa 1-306)

VAng-Lsx5831-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Campylobacter Concisus Tetraacyldisaccharide 4'-kinase, recombinant protein.

Recombinant Campylobacter Concisus lpxK Protein (aa 1-306)

VAng-Lsx5831-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Campylobacter Concisus Tetraacyldisaccharide 4'-kinase, recombinant protein.

Recombinant Campylobacter Concisus lpxK Protein (aa 1-306)

VAng-Lsx5831-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Campylobacter Concisus Tetraacyldisaccharide 4'-kinase, recombinant protein.

Recombinant Campylobacter Jejuni argF Protein (aa 1-306)

VAng-Lsx6191-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Campylobacter Jejuni Ornithine carbamoyltransferase, recombinant protein.

Recombinant Campylobacter Jejuni argF Protein (aa 1-306)

VAng-Lsx6191-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Campylobacter Jejuni Ornithine carbamoyltransferase, recombinant protein.

Recombinant Campylobacter Jejuni argF Protein (aa 1-306)

VAng-Lsx6191-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Campylobacter Jejuni Ornithine carbamoyltransferase, recombinant protein.

Recombinant Clostridium Acetobutylicum tsf Protein (aa 1-306)

VAng-Lsx7894-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Clostridium Acetobutylicum Elongation factor Ts, recombinant protein.

Recombinant Clostridium Acetobutylicum tsf Protein (aa 1-306)

VAng-Lsx7894-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Clostridium Acetobutylicum Elongation factor Ts, recombinant protein.

Recombinant Clostridium Acetobutylicum tsf Protein (aa 1-306)

VAng-Lsx7894-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Clostridium Acetobutylicum Elongation factor Ts, recombinant protein.

Recombinant Clostridium Botulinum murB Protein (aa 1-306)

VAng-Lsx8338-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Clostridium Botulinum (strain Okra / Type B1) UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein.

Recombinant Clostridium Botulinum murB Protein (aa 1-306)

VAng-Lsx8338-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Clostridium Botulinum (strain Okra / Type B1) UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein.

Recombinant Clostridium Botulinum murB Protein (aa 1-306)

VAng-Lsx8338-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Clostridium Botulinum (strain Okra / Type B1) UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein.

Recombinant Clostridium Cellulolyticum metA Protein (aa 1-306)

VAng-Lsx8962-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Clostridium Cellulolyticum Homoserine O-succinyltransferase, recombinant protein.

Recombinant Clostridium Cellulolyticum metA Protein (aa 1-306)

VAng-Lsx8962-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Clostridium Cellulolyticum Homoserine O-succinyltransferase, recombinant protein.

Recombinant Clostridium Cellulolyticum metA Protein (aa 1-306)

VAng-Lsx8962-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Clostridium Cellulolyticum Homoserine O-succinyltransferase, recombinant protein.

Recombinant Clostridium Difficile pyrB Protein (aa 1-306)

VAng-Lsx9272-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Clostridium Difficile (strain 630) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Clostridium Difficile pyrB Protein (aa 1-306)

VAng-Lsx9272-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Clostridium Difficile (strain 630) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Clostridium Difficile pyrB Protein (aa 1-306)

VAng-Lsx9272-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Clostridium Difficile (strain 630) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Helicobacter Pylori hemC Protein (aa 1-306)

VAng-Lsx3515-1mgEcoli 1 mg (E. coli)
EUR 4344
Description: Helicobacter Pylori Porphobilinogen deaminase, recombinant protein.

Recombinant Helicobacter Pylori hemC Protein (aa 1-306)

VAng-Lsx3515-500gEcoli 500 µg (E. coli)
EUR 2940
Description: Helicobacter Pylori Porphobilinogen deaminase, recombinant protein.

Recombinant Helicobacter Pylori hemC Protein (aa 1-306)

VAng-Lsx3515-50gEcoli 50 µg (E. coli)
EUR 2004
Description: Helicobacter Pylori Porphobilinogen deaminase, recombinant protein.

Recombinant Shigella Dysenteriae cbpA Protein (aa 1-306)

VAng-Lsx06787-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Curved DNA-binding protein, recombinant protein.

Recombinant Shigella Dysenteriae cbpA Protein (aa 1-306)

VAng-Lsx06787-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Curved DNA-binding protein, recombinant protein.

Recombinant Shigella Dysenteriae cbpA Protein (aa 1-306)

VAng-Lsx06787-50gEcoli 50 µg (E. coli)
EUR 2003
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Curved DNA-binding protein, recombinant protein.

Recombinant Shigella Dysenteriae speB Protein (aa 1-306)

VAng-Lsx07206-1mgEcoli 1 mg (E. coli)
EUR 4147
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Agmatinase, recombinant protein.

Recombinant Shigella Dysenteriae speB Protein (aa 1-306)

VAng-Lsx07206-500gEcoli 500 µg (E. coli)
EUR 2938
Description: Shigella Dysenteriae serotype 1 (strain Sd197) Agmatinase, recombinant protein.
The protein consists of 1136 amino acid residues encoded by 3411 bases and has a molecular weight of 125.35 kDa. The optimum temperature and pH of this enzyme are 50°C and pH 4.0. In addition, this examine confirmed that the unknown perform area (encoding Lys261-Thr681) outlined as a linker is sort of necessary for its exercise. The obtained novel enzyme BcFucA will contribute to the efficient degradation of fucoidan and future industrial functions.

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