a universal protein array system for quantitative detection of protein-protein, body weight and body mass index on patients with hypercholesterolemia: a systematic review and meta-analysis., Cotranslational protein-RNA associations predict protein-protein interactions., Diagnostic performance of circulating exosomes in human cancer: A meta-analysis., DNA, Effects of plant protein and animal protein on lipid profile, Gene, Predicting protein-protein binding sites in membrane proteins., protein-DNA, protein-RNA and protein-ligand interactions., Proteins, Reagents, RNA

Mechanistic Studies of the TRIP Catalyzed Allylation with Organozinc Reagents

Mechanistic Studies of the TRIP Catalyzed Allylation with Organozinc Reagents

3,3-Bis(2,4,6-triisopropylphenyl)-1,1-binaphthyl-2,2-diyl hydrogenphosphate (TRIP) catalyzes the uneven allylation of aldehydes with organozinc compounds resulting in extremely helpful structural motifs, like precursors to lignan pure merchandise.

Our mechanistic proposal beforehand reported depends on two response intermediates and requires additional investigation as a way to actually perceive the mode of motion and the origins of stereoselectivity. Detailed ab initio calculations, supported by experimental knowledge, render a considerably completely different mode of motion to the allyl boronate congener. Instead of a Brønsted acid primarily based catalytic activation, the chiral phosphate acts as a counterion for the Lewis acidic zinc ion, which supplies the activation of the aldehyde.

Dragendorff’s reagent: Historical views and present standing of a flexible reagent launched over 150 years in the past at the University of Dorpat, Tartu, Estonia

  • The well-known Dragendorff’s reagent (DR) was launched by an Estonian-German Professor Johann Georg Noel Dragendorff (1836-1898) in the center of the 19th century (1866). Dragendorff, who was a full-time professor in pharmacy at the college of Dorpat (Tartu) used his reagent initially for the fast screening of natural merchandise to seek out traces of alkaloids.
  • DR is an answer of potassium bismuth iodide composing of fundamental bismuth nitrate (Bi(NO₃)₃), tartaric acid, and potassium iodide (KI), and when contact with alkaloids DR produces an orange or orange crimson precipitate.
  • In this assessment article, we make a brief historic overview on the biography and scientific analysis work of Professor Dragendorff at the University of Dorpat.
  • The chemistry, methodology of preparation, mechanism of motion, and sensible makes use of of DR in varied disciplines in varied European nations together with the Baltic nations (Estonia, Latvia, Lithuania), Finland, Ukraine, Moldova, and in Asia (Vietnam), are additionally mentioned. Over a number of many years, DR and its modifications have discovered makes use of in lots of new purposes and disciplines, and a quantity of business DRs are additionally at present out there on the market.
  • Today, DR is used for instance in the manufacturing of surfactants, the place non-ionic surfactant is precipitated in water answer with modified DR (OkayBiI₄+BaCl₂+glacial acetic acid).
  • A complete six completely different potassium iodobismuthate (DR) options are additionally offered in the European Pharmacopoeia. In conclusion, DR (after greater than 150 years of its invention in Estonia) has nonetheless an necessary position in pharmaceutical and associated sciences throughout the world.

Comparative examine between a medical gadget and reagent dipsticks in measuring pH

Objectives: Urinary pH is a decisive consider a number of pathologies, thereby an informative marker employed in remedy selections. Although extensively used, the urinary pH dipstick check will not be sufficiently correct or exact for scientific selections and extra strong methodologies must be thought of. In this examine, we evaluate pH measurements when utilizing a conveyable medical gadget and completely different dipstick checks.

Materials and strategies: Four pH dipstick manufacturers and a Lit-Control® pH Meter have been examined utilizing business buffer options with seven distinct pH values representing the physiological vary in urine (4.66; 5.0;5.5; 6.0; 7.0; 7.5; 8.0). A statistical evaluation was carried out to evaluate the correlation of measured versus actual values, collectively with validity measures as decision, precision and accuracy.

Results: Validity measures said the superiority of the moveable pH meter, with a decreased dispersion of knowledge and extra actual values. Additionally, correlation evaluation demonstrates that the pH values obtained with the pH meter have been the closest to the buffers’ actual pH values.

Conclusion: The detailed comparative examine offered right here reveal the superiority of a conveyable pH meter to a number of of the most used dipstick manufacturers in the clinic. Lit-Control pH Meter represents a dependable various when monitoring of urinary pH is required, as could occur throughout the screening of illnesses or remedy monitoringin the clinic, in addition to throughout the self-monitoring the affected person below skilled supervision at dwelling.



Toward comparability of anti-drug antibody assays: is the quantity of anti-drug antibody-reagent complexes at cut-point (CP-ARC) the lacking piece?


Immunogenicity testing is a compulsory and important exercise throughout the growth of therapeutic proteins. Multiple regulatory pointers present clear suggestions on applicable immunogenicity testing methods and required bioanalytical assay performances.

Unfortunately, it’s nonetheless usually accepted {that a} comparability of the immunogenicity of completely different compounds isn’t attainable as a consequence of obvious efficiency variations of the used bioanalytical strategies.

In this attitude, we suggest the ‘cut-point anti-drug antibody-reagents complex’ (CP-ARC) idea for technical comparability of the bioanalytical strategies. The feasibility and implementation in routine assay growth is mentioned in addition to the potential enchancment of reporting of bioanalytical immunogenicity knowledge to permit comparability throughout medicine. Scientific sound comparability of the bioanalytical strategies is the first step towards comparability of scientific immunogenicity.

Sensitive detection of iron (II) sulfate with a novel reagent utilizing spectrophotometry

  • In this examine, a novel reagent was developed for delicate detection of iron (II) sulfate, spectrophotometrically. A novel thio-anthraquinone by-product, 1-(Dodecylthio)anthracene-9,10-dione (3), was synthesized from the chemical response of 1-Chloroanthraquinone (1) and 1-Dodecanethiol (2) by an authentic response methodology and was utilized in the preparation of the novel reagent known as Catal’s reagent.
  • A synthesized thio-anthraquinone analogue (3) was purified by column chromatography, and its chemical construction was characterised by spectroscopic strategies akin to Fourier-transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and ultraviolet (UV)-visible spectrophotometry.
  • The chemical and molecular construction of the developed thio-antraquinone by-product (3) was illuminated utilizing computational strategies with the GaussView5 and Gaussian09 applications. Various solvents together with ethanol, methanol, and acetonitrile have been examined in the preparation of the reagent. A focus vary from 0.2 mg mL-1as much as 10 mg mL-1 of iron (II) sulfate heptahydrate answer in distilled water was ready.
  • The absorption spectra of Catal’s reagent (0.816 mM) confirmed three peaks between 185 nm-700 nm of wavelength. However, after the response with H2O2and the 30 mM trisodium citrate dihydrate combination in the presence of an iron sulfate (II) answer, a single peak was noticed, producing a steady and reddish/brownish homogenous answer (λ max = 304 nm).
  • The following concentrations of iron (II) sulfate heptahydrate was examined utilizing developed protocol and the reagent, and the concentrations have been measured spectrophotometrically at 304 nm, 0.2-1 mg mL-1. Absorbances of response mixtures of iron (II) sulfate remained steady as much as 48 h. The outcomes indicated that the novel Catal’s reagent can be utilized for delicate spectrophotometric detection of iron (II) sulfate in aqueous options.