Production of bioactive recombinant human fibroblast growth factor 12 using a new transient expression vector in E. coli and its neuroprotective effects

Production of bioactive recombinant human fibroblast growth factor 12 using a new transient expression vector in E. coli and its neuroprotective effects
July 22, 2021 0 Comments

In latest years, an rising quantity of research have proven that fibroblast growth factor 12 (FGF12) performs necessary roles in regulating neural growth and operate. Importantly, modifications of FGF12 expression are regarded as associated to the pathophysiology of many neurological ailments. However, little analysis has been carried out to discover the protecting impact of FGF12 on nerve injury.
This examine goals to discover its neuroprotective effects using our recombinant humanized FGF12 (rhFGF12). The hFGF12 gene was cloned and ligated into an expression vector to assemble a recombinant plasmid pET-3a-hFGF12. Single colonies had been screened to acquire excessive expression engineering strains, and fermentation and purification protocols for rhFGF12 had been designed and optimized.
The organic actions and associated mechanisms of rhFGF12 had been investigated by MTT assay using NIH3T3 and PC12 cell traces. The in vitro neurotoxicity mannequin of H2O2-induced oxidative harm in PC12 cells was established to discover the protecting effects of rhFGF12. The outcomes point out that the helpful effects of rhFGF12 had been almost certainly achieved by selling cell proliferation and lowering apoptosis.
Moreover, a transgenic zebrafish (islet) with robust GFP fluorescence in the motor neurons of the hindbrain was used to determine a central harm mannequin brought on by mycophenolate mofetil (MMF). The outcomes steered that rhFGF12 may ameliorate central harm induced by MMF in zebrafish. In conclusion, we now have established an environment friendly technique to specific and purify lively rhFGF12 using an Escherichia coli expression system.
Besides, rhFGF12 performs a protecting impact of on nerve injury, and it supplies a promising therapeutic method for nerve harm. KEY POINTS: • Effective expression and purification of bioactive rhFGF12 protein in E. coli. • ERK/MAPK pathway is concerned in rhFGF12-stimulated proliferation on PC12 cells. • The rhFGF12 has the neuroprotective effects by inhibiting apoptosis.

Expression and useful identification of recombinant SARS-CoV-2 receptor binding area (RBD) from Ecoli system

The receptor binding area (RBD) of SARS-CoV-2 is situated in the C-terminal of S1 subunit of the spike (S) protein which is liable for recognizing and binding to the angiotensin-converting enzyme 2 (ACE2) receptor. The DNA encoding the SARS-CoV-2 RBD was inserted into pET-28a (+) to assemble expression plasmid pET-28a (+)/RBD. The desired RBD protein was produced in E. coli Rosetta (DE) and purified by a Ni-NTA column. The recombinant RBD was analyzed by SDS-PAGE and Western blot.
The circulation cytometry evaluation indicated that the recombinant RBD is succesful of binding to human ACE2 (hACE2) in the ACE2-overexpressed HEK293A-hACE2 cells. Our outcomes demonstrated that recombinant RBD expressed in E. coli Rosetta (DE) pressure has bioactivities and can be utilized as an antigen for analysis and as a instrument for the event of novel anti-viral medication in opposition to SASR-CoV-2.

Recombinant organophosphorus hydrolase (OPH) expression in Ecoli for the efficient detection of organophosphate pesticides

Accumulation and publicity of organophosphate pesticides are of nice concern right this moment owing to their ample utilization and potential well being hazards. Harmful effects of organophosphate pesticide publicity and limitations of the obtainable therapy strategies necessitate the event of dependable, selective, cost-effective, and delicate strategies of detection.
Production of bioactive recombinant human fibroblast growth factor 12 using a new transient expression vector in E. coli and its neuroprotective effects
We developed a novel biosensor primarily based on the enzymatic motion of recombinant organophosphorus hydrolase (OPH) expression in E. coli. We report the event of colorimetric biosensors made of His-Nus-OPH in addition to His-Nus-OPH loaded alginate microspheres. The colorimetric detection technique developed using solution-phase and alginate-encapsulated His-Nus-OPH exhibited detection limits of 0.045 and 0.039 mM, respectively, for ethyl paraoxon, and 0.101 and 0.049 mM, respectively, for methyl parathion.
Additionally, fluorescence measurement using pH-sensitive fluorescein isothiocyanate (FITC) was used to sense the amount of organophosphorus pesticides. The fluorometric detection technique using solution-phase His-Nus-OPH, with ethyl paraoxon and methyl parathion because the substrate, reveals the decrease restrict of detection as 0.014 mM and 0.044 mM, respectively.
Our outcomes exhibit the viability of His-Nus-OPH for OP detection with good sensitivity, LOD, and linear vary. We report the primary use of N-terminal His-NusA-tagged OPH, which reinforces solubility considerably and presents a important advance for the scientific neighborhood.

High cell density tradition of recombinant Ecoli in the miniaturized bubble columns

Miniaturized bubble columns (MBCs) can present mass switch traits just like stirred tank bioreactors. In this examine, a new software was developed for MBCs to research the impact of feeding technique and medium kind on the fed-batch tradition of recombinant E. coli. The outcomes confirmed that the exponential feeding technique and outlined M9 medium had been extra appropriate to attain the excessive cell density tradition (HCDC).
The most obtained cell focus in exponential feeding technique in the outlined medium with out induction, was at OD600 of 169, whereas glucose focus was maintained below 2 g/L. To the most effective of our data, this cell focus can’t be achieved in lab or pilot scale bubble columns.

DKK1 recombinant protein

E62C02401 20ug
EUR 382

PTPN1 Protein (Recombinant)

20-abx073070
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug
  • Shipped within 5-10 working days.

PPM1A Protein (Recombinant)

20-abx073071
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug
  • Shipped within 5-10 working days.

PTP4A3 Protein (Recombinant)

20-abx073078
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug
  • Shipped within 5-10 working days.

SKP1 Protein (Recombinant)

20-abx073140
  • EUR 2861.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.

Ketohexokinase Protein (Recombinant)

20-abx073142
  • EUR 2861.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug
  • Shipped within 5-10 working days.

NGAL Protein (Recombinant)

20-abx073150
  • EUR 328.00
  • EUR 3084.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

DDAH1 Protein (Recombinant)

20-abx073166
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PTP4A1 Protein (Recombinant)

20-abx073184
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PPP3R1 Protein (Recombinant)

20-abx073192
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PPM1F Protein (Recombinant)

20-abx073211
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PPME1 Protein (Recombinant)

20-abx073232
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PPP4C Protein (Recombinant)

20-abx073279
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

ACAD8 Protein (Recombinant)

20-abx073280
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

ACAT2 Protein (Recombinant)

20-abx073281
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

RNASE3 Protein (Recombinant)

20-abx073373
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

MAPKAPK3 Protein (Recombinant)

20-abx073468
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PRKAB2 Protein (Recombinant)

20-abx073469
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

ATF3 Protein (Recombinant)

20-abx073484
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

DYRK1A Protein (Recombinant)

20-abx073488
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PTPN6 Protein (Recombinant)

20-abx073509
  • EUR 3530.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

NGAL Protein (Recombinant)

20-abx073513
  • EUR 3766.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

NGAL Protein (Recombinant)

20-abx073525
  • EUR 328.00
  • EUR 4281.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

PTPMT1 Protein (Recombinant)

20-abx073545
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PPP1R14A Protein (Recombinant)

20-abx073553
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PPP1R2 Protein (Recombinant)

20-abx073582
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PRKA1RA Protein (Recombinant)

20-abx073603
  • EUR 4615.00
  • EUR 230.00
  • EUR 328.00
  • 100 ug
  • 1 µg
  • 3 µg
  • Shipped within 5-10 working days.

PPARG Protein (Recombinant)

20-abx073662
  • EUR 328.00
  • EUR 5924.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

PPP3R2 Protein (Recombinant)

20-abx073701
  • EUR 6397.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

ACADL Protein (Recombinant)

20-abx073728
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

ACADVL Protein (Recombinant)

20-abx073752
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

ACADS Protein (Recombinant)

20-abx073767
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

ACADM Protein (Recombinant)

20-abx073777
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

ACADSB Protein (Recombinant)

20-abx073808
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

PPP1R8 Protein (Recombinant)

20-abx073809
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

ATF4 Protein (Recombinant)

20-abx073858
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

CDK2 Protein (Recombinant)

20-abx073859
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

FGFR1 Protein (Recombinant)

20-abx073878
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Ribokinase Protein (Recombinant)

20-abx073896
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

ADK Protein (Recombinant)

20-abx073897
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

PTP4A2 Protein (Recombinant)

20-abx073900
  • EUR 6397.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PTPN7 Protein (Recombinant)

20-abx073941
  • EUR 230.00
  • EUR 1483.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.

PPP3CA Protein (Recombinant)

20-abx073950
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.

PPP1CA Protein (Recombinant)

20-abx073956
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.

ABHD14B Protein (Recombinant)

20-abx073961
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug
  • Shipped within 5-10 working days.

PPP1CC Protein (Recombinant)

20-abx073968
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg
  • Shipped within 5-10 working days.

PTPRC Protein (Recombinant)

20-abx073990
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg
  • Shipped within 5-10 working days.

AMH Recombinant Protein

30R-3463 100 ug
EUR 1321
Description: Anti Mullerian Hormone Antigen, Recombinant

AMH Recombinant Protein

30R-3464 100 ug
EUR 1839
Description: Anti Mullerian Hormone Antigen, Recombinant

Recombinant Protein G

7-05935 1mg Ask for price

Recombinant Protein G

7-05936 10mg Ask for price

Recombinant Protein G

7-05937 100mg Ask for price

Recombinant Protein A

DAG390 1g
EUR 1940

KIAA0513Human Recombinant Protein

PROTO60268 Regular: 10ug
EUR 317
Description: KIAA0513 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (1-401 a.a) and having a molecular mass of 47.9kDa.;KIAA0513 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Batroxobin Recombinant Protein

PROTP04971 Regular: 10ug
EUR 317
Description: The Batroxobin Recombinant Protein, produced in yeast, is a single, glycosilated polypeptide chain containing 231 amino acids and having an Mw of approximately 28-33 kDa.

Lysostaphin Recombinant Protein

PROTP10547 Regular: 5mg
EUR 366
Description: Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.;The Mw of lysostaphin is 26,921 (Recsei et al, PNAS 1987).

LIN7BHuman Recombinant Protein

PROTQ9HAP6 Regular: 10ug
EUR 317
Description: LIN7B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 230 amino acids (1-207 a.a.) and having a molecular mass of 25.3kDa.;LIN7B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Streptavidin Recombinant Protein

PROTP22629-2 Regular: 20mg
EUR 317
Description: Streptavidin Streptomyces Avidinii Recombinant produced in E.Coli. ;The molecular weight per tetramer is approximately 52kDa.

Staphylokinase Recombinant Protein

PROTP68802 Regular: 100ug
EUR 317
Description: Staphylokinase Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 136 amino acids and having a molecular weight of 16kDa.;The Staphylokinase is purified by proprietary chromatographic techniques.

TIAL1Human Recombinant Protein

PROTQ01085 Regular: 20ug
EUR 317
Description: TIAL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 398 amino acids (1-375 a.a) and having a molecular mass of 44.0kDa.TIAL1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Recombinant Coxsackievirus Protein

VAng-Lsx0074-inquire inquire Ask for price
Description: Coxsackievirus antigen, recombinant protein from E. coli.

Recombinant EBV Protein

VAng-Lsx0108-inquire inquire Ask for price
Description: EBV, recombinant protein from human cells.

Recombinant EBOV Protein

VAng-Lsx0136-1mg 1 mg
EUR 1138
Description: EBOV, recombinant protein from E. coli.

Recombinant Hantavirus Protein

VAng-Lsx0152-100gEcoli 100 µg (E. coli)
EUR 670
Description: Hantavirus, recombinant protein from E. coli.

Recombinant Hantavirus Protein

VAng-Lsx0152-1mgEcoli 1 mg (E. coli)
EUR 3402
Description: Hantavirus, recombinant protein from E. coli.

Recombinant Hantavirus Protein

VAng-Lsx0152-500gEcoli 500 µg (E. coli)
EUR 1787
Description: Hantavirus, recombinant protein from E. coli.

Recombinant RSV Protein

VAng-Lsx0448-inquire inquire Ask for price
Description: RSV, recombinant protein from Hep-2 Cells.

Recombinant HBV Protein

VAng-Lsx0175-1mg 1 mg
EUR 3470
Description: HBV, recombinant protein from E. coli.

Recombinant HDV Protein

VAng-Lsx0220-inquire inquire Ask for price
Description: HDV, recombinant protein from E. coli.

Recombinant Rotavirus Protein

VAng-Lsx0471-inquire inquire Ask for price
Description: RotaVirus, recombinant protein from MA 104 cells.

Recombinant SIV Protein

VAng-Lsx0493-inquire inquire Ask for price
Description: Recombinant SIV p55- Strains: SIV mac 23g and SIV smH4 is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells.

Recombinant Echovirus Protein

VAng-Lsx0595-inquire inquire Ask for price
Description: Echovirus, recombinant protein from E. coli.

STAG3L1 Recombinant Protein (Human) (Recombinant Tag)

RP030283 100 ug Ask for price

STAG3L2 Recombinant Protein (Human) (Recombinant Tag)

RP030286 100 ug Ask for price

STAG3L3 Recombinant Protein (Human) (Recombinant Tag)

RP030289 100 ug Ask for price

STAG3L4 Recombinant Protein (Human) (Recombinant Tag)

RP030292 100 ug Ask for price

TAGAP Recombinant Protein (Human) (Recombinant- Tag)

RP030880 100 ug Ask for price

TAGLN Recombinant Protein (Human) (Recombinant- Tag)

RP030883 100 ug Ask for price

TAGLN Recombinant Protein (Human) (Recombinant- Tag)

RP030886 100 ug Ask for price

TAGLN2 Recombinant Protein (Human) (Recombinant Tag)

RP030889 100 ug Ask for price

TAGLN2 Recombinant Protein (Human) (Recombinant Tag)

RP030892 100 ug Ask for price

TAGLN3 Recombinant Protein (Human) (Recombinant Tag)

RP030895 100 ug Ask for price

CTAG1A Recombinant Protein (Human) (Recombinant- Tag)

RP038332 100 ug Ask for price

CTAG1B Recombinant Protein (Human) (Recombinant- Tag)

RP038335 100 ug Ask for price

CTAGE5 Recombinant Protein (Human) (Recombinant- Tag)

RP038338 100 ug Ask for price

TAGAP Recombinant Protein (Human) (Recombinant- Tag)

RP043930 100 ug Ask for price

CTAGE3P Recombinant Protein (Human) (Recombinant Tag)

RP053400 100 ug Ask for price

CTAGE4 Recombinant Protein (Human) (Recombinant- Tag)

RP053403 100 ug Ask for price

CTAGE8 Recombinant Protein (Human) (Recombinant- Tag)

RP053409 100 ug Ask for price

CTAGE9 Recombinant Protein (Human) (Recombinant- Tag)

RP053412 100 ug Ask for price

CTAGE1 Recombinant Protein (Human) (Recombinant- Tag)

RP008266 100 ug Ask for price

CTAGE5 Recombinant Protein (Human) (Recombinant- Tag)

RP008269 100 ug Ask for price

CTAGE6P Recombinant Protein (Human) (Recombinant Tag)

RP008272 100 ug Ask for price

CTAGEP Recombinant Protein (Human) (Recombinant- Tag)

RP008275 100 ug Ask for price

TAGLN Recombinant Protein (Rat) (Recombinant- Tag)

RP232205 100 ug Ask for price

TAGLN2 Recombinant Protein (Rat) (Recombinant Tag)

RP232208 100 ug Ask for price

TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)

RP232211 100 ug Ask for price

TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)

RP232214 100 ug Ask for price

TAGAP Recombinant Protein (Mouse) (Recombinant- Tag)

RP177155 100 ug Ask for price

Tagap1 Recombinant Protein (Mouse) (Recombinant Tag)

RP177158 100 ug Ask for price

TAGLN Recombinant Protein (Mouse) (Recombinant- Tag)

RP177161 100 ug Ask for price

TAGLN2 Recombinant Protein (Mouse) (Recombinant Tag)

RP177164 100 ug Ask for price

TAGLN3 Recombinant Protein (Mouse) (Recombinant Tag)

RP177167 100 ug Ask for price

CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag)

RP126449 100 ug Ask for price

CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag)

RP126452 100 ug Ask for price

CTAGE5 Recombinant Protein (Mouse) (Recombinant- Tag)

RP126455 100 ug Ask for price

Protein Export Protein SecB Recombinant Protein

PROTP0AG86 Regular: 25ug
EUR 317
Description: Recombinant E.Coli SecB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids and having a molecular mass of 17.2 kDa. SecB was over-expressed in E. coli and purified by conventional chromatography.

Recombinant Protein Export Protein SecB

7-06340 5µg Ask for price

Recombinant Protein Export Protein SecB

7-06341 25µg Ask for price

Recombinant Protein Export Protein SecB

7-06342 1mg Ask for price

Zika NS1 Protein Recombinant Protein

PROTB5AHE9 Regular: 0.5mg
EUR 788
Description: The E.Coli derived Recombinant Zika NS1 protein (Strain: ZikaSPH2015) having an Mw of 45kDa is derived from the full length Zika NS1 protein. ;The Zika NS1 protein is fused to a 6xHis tag at C-terminus and purified by proprietary chromatographic technique.

p53 Protein Human Recombinant Protein

PROTP04637-1 Regular: 5ug
EUR 479
Description: p53 Human Recombinant full length produced in E.Coli is a non-glycosylated, polypeptide chain having a total Mw of 81kDa. p53 Human Recombinant is fused to GST tag and purified by proprietary chromatographic techniques.

PHF11 Protein Human Recombinant Protein

PROTQ9UIL8 Regular: 20ug
EUR 317
Description: PHF11 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 351 amino acids (1-331 a.a.) and having a molecular mass of 39.7kDa.;PHF11 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

BAD Protein Human Recombinant Protein

PROTQ92934 Regular: 5ug
EUR 479
Description: Bcl2 antagonist of cell death (BAD) Human Recombinant full length protein expressed in E.coli, shows a 51 kDa band on SDS-PAGE(Icluding GST tag). ;The BAD protein is purified by proprietary chromatographic techniques.

CTAG2 Recombinant Protein (Human) (Recombinant-P Tag)

RP053376 100 ug Ask for price

CTAG2 Recombinant Protein (Rat) (Recombinant-P Tag)

RP196619 100 ug Ask for price

CTAG2 Recombinant Protein (Mouse) (Recombinant-P Tag)

RP126446 100 ug Ask for price

Disulfide Isomerase Protein (Recombinant)

20-abx073027
  • EUR 940.00
  • EUR 370.00
  • EUR 1428.00
  • 0.5 mg
  • 100 ug
  • 1 mg
  • Shipped within 5-10 working days.

Phosphatase 1G Protein(Recombinant)

20-abx073051
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug
  • Shipped within 5-10 working days.

Deoxythymidylate Kinase Protein (Recombinant)

20-abx073454
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Pantothenate Kinase Protein (Recombinant)

20-abx073459
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Cyclin-A2 Protein (Recombinant)

20-abx073464
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Mevalonate Kinase Protein (Recombinant)

20-abx073473
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

E.coli Glucokinase Protein (Recombinant)

20-abx073476
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Phosphomevalonate Kinase Protein (Recombinant)

20-abx073487
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Cyclin-I Protein (Recombinant)

20-abx073492
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Phosphoserine Phosphatase Protein (Recombinant)

20-abx073510
  • EUR 3530.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Riboflavin Kinase Protein (Recombinant)

20-abx073520
  • EUR 3766.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Galactokinase 1 Protein (Recombinant)

20-abx073596
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Deoxycytidine Kinase Protein (Recombinant)

20-abx073597
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Phosphofructokinase, Muscle Protein (Recombinant)

20-abx073602
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

PKAr-1a Protein (Recombinant)

20-abx073605
  • EUR 4615.00
  • EUR 230.00
  • EUR 328.00
  • 100 ug
  • 1 µg
  • 3 µg
  • Shipped within 5-10 working days.

Ribosomal Protein L12 (Recombinant)

20-abx073817
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Cyclin-B2 Protein (Recombinant)

20-abx073867
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Hexokinase-1 Protein (Recombinant)

20-abx073875
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Hexokinase-2 Protein (Recombinant)

20-abx073876
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Hexokinase-3 Protein (Recombinant)

20-abx073877
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Hexokinase-4 Protein (Recombinant)

20-abx073882
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Pyridoxal Kinase Protein (Recombinant)

20-abx073893
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Cyclin-B1 Protein (Recombinant)

20-abx073999
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg
  • Shipped within 5-10 working days.

c-erbB2 Protein (Recombinant)

20-abx074013
  • EUR 829.00
  • EUR 411.00
  • EUR 537.00
  • 10 ug
  • 2 µg
  • 5 ug
  • Shipped within 5-10 working days.

Pantothenate Kinase Protein (Recombinant)

20-abx076001
  • EUR 17798.00
  • EUR 10559.00
  • EUR 1080.00
  • EUR 704.00
  • 100 mg
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 3 months.

Recombinant Green Fluorescent Protein

7-06112 10µg Ask for price

Recombinant Green Fluorescent Protein

7-06113 50µg Ask for price

Recombinant Green Fluorescent Protein

7-06114 1mg Ask for price

Recombinant Staphylococcal Protein-A

7-06496 10mg Ask for price

Recombinant Staphylococcal Protein-A

7-06497 100mg Ask for price

Recombinant Staphylococcal Protein-A

7-06498 1gram Ask for price

Recombinant Der P1 Protein

7-07081 100µg Ask for price

Recombinant Der P1 Protein

7-07082 500µg Ask for price

Recombinant Der P1 Protein

7-07083 1000µg Ask for price

Recombinant Der F1 Protein

7-07084 100µg Ask for price

Recombinant Der F1 Protein

7-07085 500µg Ask for price

Recombinant Der F1 Protein

7-07086 1000µg Ask for price

Recombinant Malaria Protein HSP70

7-07087 100µg Ask for price

Recombinant Malaria Protein HSP70

7-07088 500µg Ask for price

Recombinant Malaria Protein HSP70

7-07089 1000µg Ask for price

Recombinant LASA Z Protein

0508-001 100ug
EUR 491
Description:
  • Related to: Filoviruses
  • Applications: ELISA, WB

Recombinant Protein A/G

7-05932 1mg Ask for price

Recombinant Protein A/G

7-05933 10mg Ask for price

Recombinant Protein A/G

7-05934 100mg Ask for price

Recombinant Human ACE2 Protein

ACE2-185H-20ug 20ug
EUR 570
Description: Recombinant human ACE2 (NP_068576.1) (Met1-Ser740) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Recombinant Human ACE2 Protein

ACE2-185H-50ug 50ug
EUR 830
Description: Recombinant human ACE2 (NP_068576.1) (Met1-Ser740) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Recombinant Rotavirus Vp6inant protein

DAGA-3002 0.1 mg
EUR 1235

Recombinant Human C1QBP Protein

CTP-008 100ug Ask for price
Description: A 23.9 kDa recombinant human C1QBP un-tagged protein expressed in E. Coli.

Recombinant Human C1QL1 Protein

CTP-016 100ug Ask for price
Description: A recombinant human C1QL1 protein with a N-terminal tag or C-terminal tag expressed inE. Coli, yeast, insect, or mammalian cell.
At the top of the method, adversarial impact of the metabolic burden attributable to induction and mass switch limitations decreased the obtained remaining cell focus to OD600 of 116. Finally, a comparability of the outcomes for fed-batch tradition in the stirred tank bioreactor with these of the MBCs confirmed that their decrease cell concentrations had been because of the hydrodynamics limitations of MBCs. Yet, it was discovered that the MBCs are environment friendly instruments in growth of feeding methods and analysis of medium elements for HCDC of recombinant E. coli.

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