UPA, a universal protein array system for quantitative detection of protein-protein, protein-DNA, protein-RNA and protein-ligand interactions.
Protein–protein interactions have been broadly used to check gene expression pathways and could also be thought-about as a new method to drug discovery.
Here I report the event of a universal protein array (UPA) system that gives a delicate, quantitative, multi-purpose, efficient and straightforward know-how to find out not solely particular protein–protein interactions, but in addition particular interactions of proteins with DNA, RNA, ligands and different small chemical compounds.
(i) Since purified proteins are used, the outcomes could be simply interpreted.
(ii) UPA can be utilized a number of instances for completely different targets, making it economically reasonably priced for most laboratories, hospitals and biotechnology corporations.
(iii) Unlike DNA chips or DNA microarrays, no extra instrumentation is required.
(iv) Since the UPA makes use of lively proteins (with out denaturation and renaturation), it’s extra delicate in contrast with most current strategies.
(v) Because the UPA can analyze a whole bunch (even 1000’s on a protein microarray) of proteins in a single experiment, it’s a very efficient methodology to display screen proteins as drug targets in most cancers and different human ailments.
Recent developments have utilized a DNA array-type method to immobilize proteins on a floor for high-throughput evaluation.